Wan Zhu, Fanxia Shen, Lei Mao, Lei Zhan, Shuai Kang, Zhengda Sun, Jeffrey Nelson, Rui Zhang, Dingquan Zou, Cameron M. McDougall, Michael T. Lawton, Thiennu H. Vu, Zhijian Wu, Abraham Scaria, Peter Colosi, John Forsayeth, Hua Su
Stroke May 2017, 48 (5) 1420-1423; DOI: https://doi.org/10.1161/STROKEAHA.116.015713
AAV2-sFLT02 in situ inhibits brain arteriovenous malformations (bAVM) in model 1 (R26CreER;Eng2f/2f). A, Representative images: coronal sections—latex-perfused brain. AVM developed in angiogenic regions (rectangles) of adeno-associated viral vector serotype-2 (AAV2)-EV–injected but not in AAV2-sFLT02–injected mice. Right: Close-up views: angiogenic region. Scale bars: 1 mm (left) or 500 µm (right). B, Latex-perfused area quantification. AAV2-EV group: n=6. AAV2-sFLT02 group: n=5. *P=0.037.
Wan Zhu, Fanxia Shen, Lei Mao, Lei Zhan, Shuai Kang, Zhengda Sun, Jeffrey Nelson, Rui Zhang, Dingquan Zou, Cameron M. McDougall, Michael T. Lawton, Thiennu H. Vu, Zhijian Wu, Abraham Scaria, Peter Colosi, John Forsayeth, Hua Su
Stroke May 2017, 48 (5) 1420-1423; DOI: https://doi.org/10.1161/STROKEAHA.116.015713
Wan Zhu, Fanxia Shen, Lei Mao, Lei Zhan, Shuai Kang, Zhengda Sun, Jeffrey Nelson, Rui Zhang, Dingquan Zou, Cameron M. McDougall, Michael T. Lawton, Thiennu H. Vu, Zhijian Wu, Abraham Scaria, Peter Colosi, John Forsayeth, Hua Su
Stroke May 2017, 48 (5) 1420-1423; DOI: https://doi.org/10.1161/STROKEAHA.116.015713
Daniel L Cooke, Van Halbach, Steven Hetts, Matthew Amans, Christopher Dowd, Randall Higashida, Devon Lawson, Jeffrey Nelson, David McCoy, Helen Kim, Tomoki Hashimoto, Zena Werb, Hua Su and Zhengda Sun
Transfection of mesenchymal stem cells (MSCs) with the mouse-secreted Klotho (mSKL) gene. A, Map of lentiviral vector expressing the mSKL and enhanced green fluorescent protein (eGFP) genes. B, Map of the control lentiviral vector expressing eGFP only. C, Phase-contrast image of MSCs in culture. D, eGFP expression in Lenti-SKL-GFP-transduced MSCs. E, Western blot analysis of SKL protein expression in cell lysates and conditioned medium from transfected and nontransfected MSCs. CMV indicates cytomegalovirus; cPPT, central polypurine tract; IRES, internal ribosome entry site; LTR, long terminal repeat; psi, Ψ domain; RRE, Rev responsive element; RSV, rous sarcoma virus; SIN, self-inactivating LTR as a result of the deletion of the U3 promoter; SRCMVi, a CMV promoter plus enhancers from RSV and SV40 promoters and a human β-globin intron; SV40, simian virus 40; and WPRE, woodchuck hepatitis virus post-transcriptional regulatory element.
Mesenchymal stem cells (MSCs) overexpressing secreted Klotho (SKL) abolished monocrotaline (MCT)-induced pulmonary vascular dysfunction. A, Responses of small pulmonary arteries (PAs) to ACh. B, Responses of small PAs to sodium nitroprusside. #P<0.05 vs the MCT group. n=6. Data=means±SEM. GFP indicates green fluorescent protein.
Mesenchymal stem cells (MSCs) overexpressing secreted Klotho (SKL) attenuated monocrotaline (MCT)-induced pulmonary arterial remodeling. A, Representative hematoxylin and eosin staining images of small pulmonary artery (PA) cross sections (60–80 µmol/L) in rat lungs. B, Quantitative analysis of medial thickness. C, Lumen area in rat small PA cross sections. D, α-Smooth muscle actin (α-SMA) staining using immunohistochemical in small PA cross sections. E, Quantification of α-SMA staining. Scale bar, 20 µm. *P<0.05 vs saline; #P<0.05 vs MCT. n=6. Data=means±SEM. GFP indicates green fluorescent protein.
