Search for author "Zhe Sun"

• You have accessRestricted access

  Abstract 263: A Decline in Kidney-derived Protein Klotho Contributes to Aging-related Heart Failure

  Zhongjie Sun and Kai Chen

  Circulation Research. 2016;119:A263
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 1.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Western diet (WD)–induced endothelial cell (EC) stiffness and aortic relaxation dysfunction is preve...

  Show More

  Western diet (WD)–induced endothelial cell (EC) stiffness and aortic relaxation dysfunction is prevented in EC mineralocorticoid receptor (ECMR) ^−/− female mice. A, Aortic pulse wave velocity (PWV) measured in vivo after 16-week feeding trial on WD. Values are mean±SE; n=6 to 10 per group. B, The ex vivo measurement of endothelium stiffness by using atomic force microscopy; n=4 to 5 per group. C, A representative deflection image in EC shows clear stress fibers in the cytoskeleton. D, ECMR^−/− prevented aldosterone treatment (10⁻⁸ mol/L) and induced cultured EC stiffness in vitro. Values are mean±SE; n=6 per group. *P<0.05 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 2.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Endothelial cell mineralocorticoid receptor (ECMR) mediates endothelial sodium channel (ENaC) expres...

  Show More

  Endothelial cell mineralocorticoid receptor (ECMR) mediates endothelial sodium channel (ENaC) expression in Western diet (WD)–induced aortic relaxation dysfunction. WD ECMR^+/+ increased MR expression (A), which bind the hormone-response element (nGnACAnnnTGTnCn) on the site of ENaC promoter (B), and prompted an increase in ENaC expression (C). ECMR^−/− prevented WD (C) and aldosterone (D)-induced expression of ENaC in vivo and in vitro, respectively. Representative images immunostaining for ENaC (E) and serum- and glucocorticoid-regulated kinase 1 (F) in ECs with corresponding measures of average gray scale intensities. Scale bar, 50 μm; n=4 to 5 per group. *P<0.01 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 3.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  In vivo amiloride administration ameliorated Western diet (WD)–impaired aortic relaxation and flow-i...

  Show More

  In vivo amiloride administration ameliorated Western diet (WD)–impaired aortic relaxation and flow-induced mesenteric artery dilation in endothelial cell mineralocorticoid receptor (ECMR)^+/+ female mice. Vasodilator responses of isolated aortic rings to the endothelium-dependent dilators, acetylcholine (A) and to the endothelium-independent vasodilator, sodium nitroprusside (B). ECMR^−/− (C) and amiloride (D) improved flow-induced mesenteric artery dilation in mice fed a WD. n=4 to 5 per group. *P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 4.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Endothelial cell mineralocorticoid receptor (ECMR)^−/−prevents Western d...

  Show More

  Endothelial cell mineralocorticoid receptor (ECMR)^−/− prevents Western diet (WD)–impaired endothelial nitric oxide synthase (eNOS) activation and endothelial-dependent aortic relaxation. Vasodilator responses of isolated aortic rings to the endothelium-dependent dilators, acetylcholine (A) and insulin (B). C, The expression and activation of protein kinase B (Akt) and eNOS were performed with immunoblotting. D, Quantitative analysis of protein expression in p-Akt and p-eNOS; n=4 to 5 per group. *P<0.05 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 5.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Western diet (WD)–induced aortic oxidative stress and maladaptive proinflammatory cytokines are amel...

  Show More

  Western diet (WD)–induced aortic oxidative stress and maladaptive proinflammatory cytokines are ameliorated in endothelial cell mineralocorticoid receptor (ECMR)^−/− female mice. A, Western blot for 3-nitrotyrosine, a marker of oxidant stress from accumulation of oxidant peroxynitrite (ONOO⁻). B, mRNA expression of Nox2 and p22phox in aortic tissues. C, Expression of M1/M2 macrophage marker CD86, CD11c, CD206, and interleukin (IL)10. D, WD ECMR^−/− increased M2 macrophage marker IL10 and CD206 mRNA expression in WD-fed mice as measured by real-time PCR; n=4 per group. *P<0.01 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 6.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Western diet (WD)–induced aortic remodeling and ultrastructural abnormalities are prevented in endot...

  Show More

  Western diet (WD)–induced aortic remodeling and ultrastructural abnormalities are prevented in endothelial cell mineralocorticoid receptor (ECMR)^−/− female mice. Representative micrographs show medial wall thickening staining with Verhoeff–Van Gieson (A) and periaortic fibrosis staining with picrosirius red staining (B). C, ECMR^−/− prevents WD-induced increases in thickened electron dense plasmalemma and free ribosomes, which contributed to EC stiffness. Magnification, ×10 000; scale bar, 0.5 µm. D, ECMR^−/− prevents WD-induced upregulation of p-extracellular signal–regulated kinase 1/2; n=4 to 6 per group. *P<0.05 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have accessRestricted access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Circulation Research. 2016;118:935-943, originally published February 15, 2016

  https://doi.org/10.1161/CIRCRESAHA.115.308269

  Download PDF
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 1.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Western diet (WD)–induced endothelial cell (EC) stiffness and aortic relaxation dysfunction is preve...

  Show More

  Western diet (WD)–induced endothelial cell (EC) stiffness and aortic relaxation dysfunction is prevented in EC mineralocorticoid receptor (ECMR) ^−/− female mice. A, Aortic pulse wave velocity (PWV) measured in vivo after 16-week feeding trial on WD. Values are mean±SE; n=6 to 10 per group. B, The ex vivo measurement of endothelium stiffness by using atomic force microscopy; n=4 to 5 per group. C, A representative deflection image in EC shows clear stress fibers in the cytoskeleton. D, ECMR^−/− prevented aldosterone treatment (10⁻⁸ mol/L) and induced cultured EC stiffness in vitro. Values are mean±SE; n=6 per group. *P<0.05 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less
• You have access

  Endothelial Mineralocorticoid Receptor Mediates Diet-Induced Aortic Stiffness in FemalesNovelty and Significance

  Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe, James R. Sowers

  Circulation Research March 2016, 118 (6) 935-943; DOI: https://doi.org/10.1161/CIRCRESAHA.115.308269

  

  Figure 2.

  By Guanghong Jia, Javad Habibi, Annayya R. Aroor, Luis A. Martinez-Lemus, Vincent G. DeMarco, Francisco I. Ramirez-Perez, Zhe Sun, Melvin R. Hayden, Gerald A. Meininger, Katelee Barrett Mueller, Iris Z. Jaffe and James R. Sowers

  Endothelial cell mineralocorticoid receptor (ECMR) mediates endothelial sodium channel (ENaC) expres...

  Show More

  Endothelial cell mineralocorticoid receptor (ECMR) mediates endothelial sodium channel (ENaC) expression in Western diet (WD)–induced aortic relaxation dysfunction. WD ECMR^+/+ increased MR expression (A), which bind the hormone-response element (nGnACAnnnTGTnCn) on the site of ENaC promoter (B), and prompted an increase in ENaC expression (C). ECMR^−/− prevented WD (C) and aldosterone (D)-induced expression of ENaC in vivo and in vitro, respectively. Representative images immunostaining for ENaC (E) and serum- and glucocorticoid-regulated kinase 1 (F) in ECs with corresponding measures of average gray scale intensities. Scale bar, 50 μm; n=4 to 5 per group. *P<0.01 compared with CD ECMR^+/+; #P<0.05 compared with WD ECMR^+/+.

  Show Less

Pages