Cross-lagged path analysis of childhood body mass index (BMI) and insulin (n=990), adjusted for age, race, sex, and follow-up years. β1 and β2 are cross-lagged path coefficients; r1 is synchronous correlations; r2 and r3 are tracking correlations; R2 is variance explained. Goodness-of-fit: root mean square residual=0.063 and comparative fit index=0.93. Coefficients different from 0: *P<0.01, †P<0.001 for difference between β1 and β2.
Cross-lagged analysis models of childhood body mass index (BMI) and insulin in normotensive and hypertensive groups, adjusted for age, race, sex, and follow-up years. β1 and β2 are cross-lagged path coefficients; r1 is synchronous correlations; r2 and r3 are tracking correlations; R2 is variance explained. Goodness-of-fit: root mean square residual (RMR)=0.047 and comparative fit index (CFI)=0.94 for hypertensives; RMR=0.069 and CFI=0.93 for normotensives; Coefficients different from 0: *P<0.01, †P<0.001 for difference in β2s between hypertensive and normotensive groups.
By Shi‐qunSun, TuoZhang, DingDing, Wei‐fengZhang, Xiao‐leiWang, ZheSun, Liu‐huaHu, Sheng‐yingQin, Ling‐hongShen and BenHe
Selection of candidate circulating miRNAs as early biomarkers for CI‐AKI in rats. A, Global miRNA expression profiles of kidney and plasma in CI‐AKI a...Show More
Selection of candidate circulating miRNAs as early biomarkers for CI‐AKI in rats. A, Global miRNA expression profiles of kidney and plasma in CI‐AKI and control rats by miRNA microarrays. Principal component analysis (PCA) revealed that the hybridization results of 2 plasma samples (1 from the CI‐AKI group and 1 from the control group) were significantly different from the other plasma samples. These 2 components explained 81.19% of the variability. B, Hierarchical clustering analysis of dysregulated miRNAs (with a fold change >1.5) in plasma and kidney samples. The relative miRNA expression is depicted according to the color scale shown on the right. Red indicates upregulation and blue, downregulation. C, Diagram of the strategy for identifying candidate miRNAs. See Results for details. CI‐AKI indicates contrast‐induced acute kidney injury; CM, contrast medium; miRNA, microRNA; NS, normal saline; PCA, principal component analysis.Show Less
Validation of candidate circulating miRNAs in rats. A, Tissue expression patterns of miRNAs in rats. The levels of candidate miRNAs in various tissues from rats by reverse transcription quantitative real‐time PCR (RT‐qPCR). B, Validating data of miRNA microarray by RT‐qPCR method. C, Time course of the plasma levels of candidate miRNAs from CI‐AKI rats. The plasma was collected from the rats at different time points (n=4 for each time point). Levels of miRNAs at each time point were expressed as fold increase relative to the baseline. D, The plasma was collected from control rats (without pretreatment and CM exposure), CM‐only group (CM exposure without pretreatment), dehydration‐only group (dehydration pretreatment without CM exposure), and CI‐AKI group at 4 hours after CM exposure or equal time point (n=4, each group). The relative change of miRNA levels is expressed as fold increase compared with the control.*P<0.05, **P<0.01, and ***P<0.001. CI‐AKI indicates contrast‐induced acute kidney injury; CM, contrast medium; miRNA, microRNA; RT‐qPCR, reverse transcription quantitative real‐time polymerase chain reaction.
