Characterization of the heart failure (HF) model. A, Echocardiography of the left side of heart in sham-operated and HF animals during diastole and sy...Show More
Characterization of the heart failure (HF) model. A, Echocardiography of the left side of heart in sham-operated and HF animals during diastole and systole. B, Right side of heart from sham-operated and HF animals; right atrium is outlined by dashed line. C, Relative abundance of NHE1 and Tbx3 transcripts in AM and SAN in sham-operated and HF animals. Data are presented as mean±SEM (n=7). AM indicates atrial muscle; LA, left atrium; LV, left ventricle; NHE1, Na+-H+ exchanger; SAN, sinoatrial node; Tbx3, T-box transcription factor. *Significantly different (false discovery rate) from corresponding data from sham-operated animals.Show Less
HF-dependent changes in SAN function. A, Mean±SEM (n=10) normal heart rate (in vivo) and intrinsic heart rate (in vitro) in sham-operated and HF anima...Show More
HF-dependent changes in SAN function. A, Mean±SEM (n=10) normal heart rate (in vivo) and intrinsic heart rate (in vitro) in sham-operated and HF animals. B, ECG recordings from sham-operated and HF animals (anesthetized). C, ECG-like recordings from Langendorff-perfused hearts from sham-operated and HF animals. Measurement of BCL and PQ interval are shown. D, Mean±SEM (n=10) SNRTc in sham-operated and HF animals. E, ECG-like recordings from Langendorff-perfused hearts from sham-operated and HF animals showing recording of SNRT. Final paced beats (10-s pacing) are shown (stimuli indicated by ▴). SNRT was time to first SAN beat after train. BCL indicates basic cycle length; SNRT, SAN recovery time; SNRTc, corrected SAN recovery time. Other abbreviations as in Figure 1. *Significantly different from corresponding data from sham-operated animals.Show Less
Relative abundance of Na+ (A) and Ca2+ (B) channel transcripts in AM and SAN in sham-operated and HF animals. Relatively poorly expressing transcripts...Show More
Relative abundance of Na+ (A) and Ca2+ (B) channel transcripts in AM and SAN in sham-operated and HF animals. Relatively poorly expressing transcripts are shown on the left, and more highly expressed transcripts are shown on the right; dotted line separates the 2 groups. Data are presented as mean±SEM (n=7). Abbreviations as in Figure 1. *Significantly different (false discovery rate) from corresponding data from sham-operated animals.Show Less
A and B, Relative abundance of K+ channel transcripts in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. Abbreviations as in...Show More
A and B, Relative abundance of K+ channel transcripts in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. Abbreviations as in Figure 1.Show Less
Relative abundance of HCN (A), TRPC (B), and connexin (C) transcripts in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. HCN...Show More
Relative abundance of HCN (A), TRPC (B), and connexin (C) transcripts in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. HCN indicates hyperpolarization-activated cyclic nucleotide-gated channels; TRPC, transient receptor potential channels. Other abbreviations as in Figure 1.Show Less
Relative abundance of Ca2+-handling transcripts in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. IP3 indicates inositol 1,4...Show More
Relative abundance of Ca2+-handling transcripts in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. IP3 indicates inositol 1,4,5-trisphosphate; NCX1, Na+-Ca2+ exchanger; PMCA1, plasma membrane Ca2+ pump isoform 1; RYR3, ryanodine receptor 3; SERCA2a, sarcoplasmic reticulum Ca2+ ATPase. Other abbreviations as Figure 1.Show Less
Relative abundance of transcripts for pumps, exchangers, and cotransporters (A); receptors (B); and miscellaneous proteins (C) in AM and SAN in sham-o...Show More
Relative abundance of transcripts for pumps, exchangers, and cotransporters (A); receptors (B); and miscellaneous proteins (C) in AM and SAN in sham-operated and HF animals. See Figure 3 legend for key. AE indicates Cl−-HCO3− exchanger; ANP, atrial natriuretic peptide; BNP, brain natriuretic peptide; NBC, Na+-HCO3− cotransporter; MCT1, monocarboxylate transporter 1; MHC, myosin heavy chain. Other abbreviations as in Figure 1.Show Less
