Low-Dose Exposure to Ionizing Radiation Deregulates the Brain-Specific MicroRNA-134 in Interventional Cardiologists
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Editorial, see p 2417
In recent years, there has been growing concern about the health risks for contemporary interventional cardiologists who have high and unprecedented levels of occupational ionizing radiation exposure.1
MicroRNAs (miRNAs), a class of short and highly conserved noncoding RNA molecules (≈22 nucleotides), have been shown to become dysregulated in many human diseases.2 The stability and tissue specificity of circulating miRNAs make them ideal biomarkers to explore the molecular mechanisms underlying the association between chronic low-dose radiation exposure and potential disease risk. The purpose of this study was to apply a miRNome-wide microarray to characterize the plasma miRNA profiles in interventional cardiologists professionally exposed to ionizing radiation.
The study consisted of 3 stages: discovery, selection, and validation. In the discovery stage, a microarray analysis (Agilent Human miRNA Microarray 8×60K Release 19.0 platform containing 2006 human miRNA probes) was performed on plasma from 10 interventional cardiologists (6 men; age, 54±5.4 years) and 10 age- and sex-matched unexposed control subjects (6 men; age, 53.2±5.5 years).
In the selection stage, all dysregulated miRNAs were evaluated in another 80 plasma samples (40 from interventional cardiologists and 40 from unexposed subjects) by quantitative real-time polymerase chain reaction. …