Abstract 20716: Can Long-Term Endurance Exercise Change MicroRNA Profiles and Prevent Cardiovascular Disease?
Introduction: Exercise improves cardiovascular fitness and reduces the incidence of cardiovascular disease (CVD). MicroRNAs (miR) are small non-coding RNA molecules that regulate gene expression and cardiovascular physiology. We investigated the effects of long-term endurance exercise on microRNA signatures in leukocytes.
Methods: We sequenced total microRNAs from leukocytes of six athletes and six healthy age-matched controls using the Illumina TruSeq Small RNA library prep on MiSeq.
Results: We found eight microRNAs (miR-17, 25, 27, 140, 142, 148, 191 and 423) differentially expressed between athletes and controls (-0.44<FC<1.17; FDR<0.1). miR-17 which is downregulated in patients with CVD was found to be upregulated (FC=0.48; P<0.01) in our athlete group. miR-27 known to induce hypertrophic cell growth was found to be downregulated (FC=-0.57; P<0.01) in athletes. Changes in these microRNAs could be a cause of cardioprotection caused by exercise. Furthermore, we found 7 novel microRNAs. Three novel microRNAs were only found in athletes and one in controls. The possible targets for these microRNAs are still unknown and need to be investigated.
Conclusions: In conclusion, long-term endurance exercise influences microRNA profiles helping prevent or protect against CVD.
Author Disclosures: P.R. Prestes: None. B.J. O’Brien: None. F.J. Charchar: None.
- © 2016 by American Heart Association, Inc.