Abstract 20232: Circulating MicroRNAs Associated With Cardiovascular Disease Do Not Improve Prediction of Incident Cardiovascular Events Within Five Years in Asymptomatic Individuals
Introduction: To investigate if circulating microRNAs (miRNAs) have utility as markers for incident cardiovascular events in the asymptomatic general population, we had previously screened plasma levels of 10 miRNAs associated with myocardial infarction (miR-126 -197 -223), unstable plaque (miR-127 -133a -145) or stable coronary artery disease (miR-142 -146a -323a -652) in 71 asymptomatic individuals (37 with subsequent cardiovascular events within 1 year, 34 remaining event-free for more than 5 years). Here we aimed to replicate our previous finding that 9 out of 10 of these miRNAs improved prediction of incident cardiovascular events in a larger population cohort, including all participants who had an event within 5 years of sampling and matched controls who remained event-free over the same period.
Methods: miRNA levels were measured in 296 individuals from a community-based cohort (n=148 with cardiovascular events within 5 years, n=148 event-free, mean age=75±10 years and 65% male in both groups, median follow-up=3.8 years). Associations between miRNA levels, established risk factors and incident cardiovascular events (including myocardial infarction, heart failure, stroke) were tested with ANOVA and Cox regression.
Results: In contrast to our previous screen, in univariate analyses none of the miRNAs differed between individuals who had a cardiovascular event and those who remained event-free, nor were they associated with time to event. In multivariate analysis only miR-142 was associated with incident cardiovascular events, independent of age, sex, body mass index, systolic blood pressure, type 1 or 2 diabetes, cigarette smoking, physical activity and plasma levels of the cardiac marker, NT-proBNP (hazard ratio [95% confidence interval] above/below median miR-142: 1.5 [1.1-2.1] p=0.023).
Conclusions: We identified a weak association between circulating levels of miR-142 and subsequent cardiovascular events within 5 years in this cohort. However, in contrast to our screen in extreme phenotype groups, these data do not support that miRNAs previously associated with cardiovascular disease improve prediction of incident cardiovascular events within 5 years in older aged asymptomatic individuals from the general population.
Author Disclosures: A.P. Pilbrow: None. C.M. Frampton: None. A.M. Richards: None. R.W. Troughton: None. V.A. Cameron: None.
- © 2016 by American Heart Association, Inc.