Abstract 19673: The Association Between Peripheral Blood Levels of B Regulatory Lymphocytes and Clinically Unstable Coronary Artery Disease
Background: Inflammatory processes within the atherosclerotic plaque are related to plaque instability with consequent acute coronary syndromes (ACS). The levels and the activity of several anti-inflammatory lymphocyte subpopulations are lower in patients with ACS. The anti-inflammatory role of B-regulatory lymphocytes (B-regs) was demonstrated in several autoimmune diseases yet their role in atherosclerosis was only examined in animal models with inconclusive results.
Purpose: we studied for the first time, levels of circulating B-regs in patients with Acute myocardial infarction (AMI) and with Stable angina pectoris (SAP).
Methods: We examined circulating levels of several subgroups of B-regs in 24 patients with recent ST-segment elevation MI (mean age 61.5±8.5 years, 88% male), 18 patients with stable angina (mean age 64.8±6, years, 94.4% male) and 26 volunteers (mean age 38±13 years, 50% male) with no evidence of coronary artery disease by flow cytometry. In 9 patients, we re-assessed Bregs levels on average 3 months later.
Results: The mean level of CD20+ cells in patients with myocardial infarction was 6.96±2.6%, 6.79±3.3% in patients with stable angina and 7.2±2.1% in healthy individuals, p=0.65. The level of CD24+CD38+ cells among those found CD20+, was 43.2±9.7% in healthy individuals, 46.1±13% in patients with stable angina, while it was as low as 24.9±13.3% in patients with myocardial infarction (p<0.001). No significant difference was found between patients with myocardial infarction, SA and healthy individuals regarding CD24+Cd27+ cells and CD1+CD5+ cells (p= 0.75 and p=0.06, respectively). On logistic regression, after correcting for age, gender, presence of hypertension, diabetes mellitus and hyperlipidemia, only the level of CD24+CD38+ Bregs were related to the presence of acute mi (OR 0.9 95%CI 0.847-0.951, p<0.001). Circulating levels of CD24+CD38+B-regs in patients with AMI did not change significantly after several months (P=0.36).
Conclusions: In conclusion circulating B-regs are reduced in patients with AMI as compared to patients with SAP. This finding may shed further light on the pathophysiologic processes underlying plaque destabilization.
Author Disclosures: I. Volodarsky: None. D. Haberman: None. S. Shimoni: None. Y. Fabrikant: None. A. Zalik: None. J. George: None.
- © 2016 by American Heart Association, Inc.