Abstract 18758: Epigenetic Regulation of Acute Coronary Syndromes
Background: Long noncoding RNAs (lncRNA) are long transcripts of more than 200 nt of length not encoding for proteins; they represent a novel source of molecules with a regulatory function at transcriptional and epigenetic level; only few information are available on their role in cardiology. As different mechanisms and prognosis have been described between type 2 Diabetic (T2DM) vs NotDiabetics patients with NSTEMI, with sought to assess whether LncRNA are involved in the different phenotypes in patients with and without diabetes.
Material and Methods: Platelet RNA from 3pts with NSTEMI without diabetes, 3 NSTEMI T2DM and 3 controls (CTRL ) were isolated for lncRNA and mRNA microarray study (ARRAYSTAR Inc USA).
Results: Global transcriptomic analyses detected 20.642 lncRNA and 18.949 mRNA in our three cohort of patients; we considered a p<0.001 and a fold induction >3. 5 as significant sign of up or down-regulation. In particular we found significant dysregulation in: a)14 lncRNA in NSTEMI vs CTRL(9 up-regulated, 5 dysregulated), b)16 lncRNA, (6 up-regulated, 10 down-regulated) in NSTEMI T2DM vs CTRL and c) 356 lncRNA,(168 up-regulated, 188 down-regulated) in NSTEMI vs NSTEMI T2DM. Moreover,using the same definition of dysregulation), we found 8 mRNA,(4 up-regulated and 4 down-regulated), significantly dys-regulated in NSTEMI vs CTRL, 16 mRNA, (10 up-regulated, 6 down-regulated), in NSTEMI T2DM vs CTRL and 109 mRNA,(51 up-regulated, 58 down-regulated), in NSTEMI vs NSTEMI T2DM.. Dys-regulated transcripts were involved in calcium signalling pathway, fibrinolysis and platelet activation.
Conclusion: Our study suggests that LncRNA are pivotal player in NSTEMI because of the presence of an important dys-regulation at epigenetic level between patients with NSTEMI and CTRL; surprisingly, the larger differences were found between NSTEMI pts with and without diabetes, suggesting an important epigenetic affect in NSTEMI with T2DM .
Author Disclosures: L.M. Biasucci: Research Grant; Significant; astra-zeneca. Speakers Bureau; Modest; siemens. M. Claudia: None. M. Grimaldi: None. G. La Rosa: None. F. Crea: None.
- © 2016 by American Heart Association, Inc.