Abstract 18750: Increased Brown Adipose Tissue as a Novel Mechanism Mediating Cardioprotection
The regulator of G protein signaling 14 (RGS14) knock out (KO) mice have a novel feature, i.e., increased brown adipose tissue, which is known to protect against cold exposure, diabetes and obesity, but less is known about its role in cardioprotection. Accordingly we examined the effects of acute myocardial ischemia induced by 30 min coronary artery occlusion (CAO) and 24 hours of coronary artery reperfusion (CAR) in 3-4 month old RGS14 KO and their wild type (WT) littermates. Infarct size, as a fraction of the area at risk determined by TTC staining, was significantly reduced, p<0.05, in RGS14 KO (27.9±0.8%), compared to WT (46.3±1.0%). The next goal of this study was to determine whether the mechanism of protection involved brown adipose tissue. Accordingly, we removed the interscapular brown adipose tissue from the RGS14 KO and transplanted it to the abdominal cavity of the WT. Two months later, both the brown adipose tissue donor (RGS14 KO) and recipient mice (WT which received the brown adipose tissue) were subjected to the CAO/CAR protocol. Under these conditions the results were reversed, i.e., infarct size was reduced in the brown adipose recipient WT mice (30.9±4.0%), but not in the RGS14 KO donors, where infarct size was now similar to that in WT mice without the brown adipose tissue transplant (40.3±3.7%). Thus, the RGS14 KO mice are protected from myocardial ischemia with a novel mechanism of brown adipose tissue mediating the cardioprotection.
Author Disclosures: J. Zhang: None. D.E. Vatner: None. S.F. Vatner: None.
- © 2016 by American Heart Association, Inc.