Abstract 18696: microRNAs Dysregulated in African Americans With Severe Hypertension: Evidences From the Mh-grid Study
Introduction: A growing body of evidence demonstrates that miRNA are key players in epigenetic regulation. Because of their regulatory role miRNAs are involved in nearly all physiological and pathophysiological processes and their dysregulation can hence contribute to disease development. Therefore miRNAs have potential therapeutic relevance and studies of miRNA expression could ultimately lead to the discovery of biomarkers for diagnosis, prognosis and treatment of diseases.
Hypothesis: Some miRNAs involved in the regulation of the cardiovascular system might be differentially expressed between subjects with severe hypertension (cases) and those with normal blood pressure (controls) . The extreme phenotype (severe hypertension) could allows us to detect signals not identified previously.
Methods: Data are from the MH-GRID project, a study of severe hypertension in African Americans aged 30 to 55 years . We conducted miRNA differential expression analysis, looking at the expression levels of 2818 miRNAs, between 78 cases and 101 controls. The expression data were normalized, using the weighted trimmed mean of M-values method and miRNAs that did not achieve 1 count per million in at least 3 samples across all samples were excluded. The analysis was adjusted for known hypertension risk factors.
Results: We identified 47 miRNAs with a significant difference in expression between severe hypertensive subjects and controls. In a separate but complementary analysis of mRNA expression from the same samples we identified 4 mRNAs, differentially expressed between cases and controls, that are regulated by 10 of the 47 miRNAs we identified in the miRNA analysis.
Conclusions: One of the mRNA regulated by miRNAs we identified is UCP2 also known as SLC25A8, a member of the solute carrier (SLC) gene family ; polymorphisms in some members of this gene family have been linked with hypertension and blood pressure.
Author Disclosures: A. Gaye: None. S. Davis: None. A. Davis: None. S. Sen: None. G. Gary: None.
- © 2016 by American Heart Association, Inc.