Abstract 18333: Association Between Leukocyte Telomere Length and Peripheral Arterial Disease: Results From NHANES
Introduction: Leukocyte telomere length (LTL), a marker of cellular senescence, may predict susceptibility to cardiovascular disease (CVD). Peripheral arterial disease (PAD) is associated with an increased risk of stroke, myocardial infarction, and death. The association between ankle brachial index, a non-invasive marker of PAD, and telomere length is not well established. We evaluated this association of ankle brachial index with LTL in community dwelling population in United States using data from National Health and Nutritional Examination Survey (NHANES) 1999-02.
Methods: LTL was measured using quantitative polymerase chain reaction method which measured telomere length relative to standard reference DNA (T/S ratio). The association of LTL with PAD (defined as ABI ≤0.9 and ABI ≥ 1.3) was assessed in general linear regression models that adjusted for conventional and novel cardiovascular risk factors, and accommodated the complex sampling design of NHANES by incorporating strata and PSU indicators.
Results: Of the 4872 participants who had ABI values measured in NHANES, telomere length data were available on 4322 participants. Mean telomere length was shorter in those with PAD (5521.6 ± 40.4 bp), and borderline 0.9<ABI≤1 (5601.6 ± 59.7 bp), than those with normal ABI (5703 ± 37.7 bp); P <.0001. A higher percentage of participants with PAD (36%) and borderline ABI (31.7%) were in the lowest quartile for telomere length than those with normal ABI (23.1%; P <0.0001). Log- transformed telomere length was associated with the presence of PAD in age and gender-adjusted models (r=-0.006 ±0.003; P=0.04). However in multivariate models that adjusted for age, gender, ethnicity, socioeconomic status, smoking, diabetes, body mass index, diabetes, emphysema/chronic bronchitis, self-reported CVD, chronic kidney disease and c-reactive protein levels, presence of PAD or borderline ABI was not associated with shorter telomere length (r= -0.001±0.005; P=0.81).
Conclusion: Although, participants with PAD in a nationally representative cross-sectional sample had lower LTL, this association was not independent of other cardiovascular risk factors. Prospective studies are needed to evaluate causal relationship between LTL and atherosclerosis.
Author Disclosures: P. Malhotra: None. N. Parikh: None. I. Kullo: Research Grant; Significant; NHGRI. S. Singh: None.
- © 2016 by American Heart Association, Inc.