Abstract 18251: Differential DNA Methylation in Coronary Artery Restenosis After Angioplasty
Introduction: Coronary artery in-stent restenosis (ISR) occurs in 20 to 30% of those who receive bare metal stents and 5 to 10% with drug eluted stents. Since known clinical and genetic risk factors do not explain all incidence aspects of ISR, the epigenetics field appears as a promising arena. Among epigenetic mechanisms, DNA methylation is the most studied and better understood, being linked to cardiovascular disease.
Hypothesis: Certain cytosine-guanine dinucleotide (CpG) sites are differentially methylated in patients who develop ISR with respect to a control group.
Methods: Forty eight patients with coronary artery stenosis ≥ 50% in the angioplasty site were included as cases and 48 with <50% stenosis as controls. DNA samples were obtained from whole blood and methylation status for ~450.000 CpG sites was measured with the Infinium HumanMethylation450 BeadChip array. The obtained values were analyzed by Student’s t test using Bonferroni correction and logistic regression (LR) adjusting for age and gender. A corrected p value of < 1х10-7 was considered statistically significant.
Results: Mean age was 64 in cases and 64.5 in controls (p=0.7), and male sex was present in 73% and 81% respectively (p=0.3). Five CpG sites were identified as hypomethylated in cases with respect to controls in genes FLAD1 (respiratory chain complex), TBL1XR1 (transcriptional activation), HDAC4 (Histone deacetylation), MASP2 (complement system activation) and AGPAT3 (lipid metabolism). See Table 1.
Conclusions: The differentially methylated CpGs found in this study can be considered as possible candidates for new epigenetic biomarkers of ISR.
Author Disclosures: F. Lanas: None. J. Ruedlinger: None. N. Saavedra: None. B. Bobadilla: None. M. Potthoff: None. L. Perez: None. L. Salazar: None.
- © 2016 by American Heart Association, Inc.