Abstract 17687: Myocardial Oxidative Stress, Urinary 8-hydroxy-2’-deoxyguanosine, is Associated With Sustained Ventricular Tachycardia in Patient With Active Cardiac Sarcoidosis
Background: Recently, we reported that urinary 8-hydroxy-2’-deoxyguanosine (U-8-OHdG), an oxidative stress marker, reflected the inflammatory activity of cardiac sarcoidosis (CS). We investigated whether U-8-OHdG levels were associated with sustained ventricular tachycardia (SVT) in patients with CS.
Methods and Results: Thirty four consecutive patients (NYHA;1.9±0.6, LVEF 38 ± 12%) were enrolled with a diagnosis of active CS based on abnormal accumulation in the heart by 18F-FDGPET/CT. These patients were divided into two groups: patients with SVT (SVT; n=19) and patients without SVT (non-SVT; n=15). A cohort of patients with a diagnosis of idiopathic delated cardiomyopathy (DCM) were used as controls (n=40). BNP, other biomarkers, and indices of cardiac function were measured in both CS and DCM patients. U-8OHdG level (ng/mg-Cr) in CS patients was significantly higher than in DCM patients (20.5±7.5 vs 11.1±3.2 P<0.0001), irrespective of no differences in indices of cardiac function. U-8-OHdG level in SVT was significantly higher than that in non-SVT (24.6±7.1 vs 15.2±3.8, P<0.0001), while there was no difference in the indices of cardiac function, renal function, SUV max between both group. Univariate and multivariate analysis showed that U-8OHdG was an independent determinant factor for SVT. ROC analysis for identification of partients with VT showed the cut-off values of U-8OHdG for VT was 17.5 ng/mg-Cr (sensitivity;89%, specificity;87%, AUC;0.92). Immunohistochemical examination of left ventricle autopsy samples revealed that positive 8-OHdG staining ratio in cardiomyocytes in SVT was significantly stronger than in non-SVT and DCM, and that U8-OHdG was significantly correlated with positive 8-OHdG staining ratio (n=50, R=0.65, p<0.01).
Conclusions: These findings suggested that U8-OHdG level, which represented myocardial oxidative stress, was associated with SVT in patients with active CS by 18F-FDG-PET. Therefore, U8-OHdG level may provide additive and relevant tissue information about arrhythmic substrate.
Author Disclosures: H. Ishiguchi: None. S. Kobayashi: None. M. Kohno: None. T. Nanno: None. T. Myoren: None. S. Oda: None. H. Tateishi: None. M. Mochizuki: None. T. Oda: None. J. Yamada: None. T. Okamura: None. M. Yano: None.
- © 2016 by American Heart Association, Inc.