Abstract 17598: Inflammatory Effect of Fasting and Postprandial Triglycerides by Leukocyte Activation
Introduction: Atherosclerosis is characterized by systemic inflammation in which the activation of leukocytes plays a crucial role. While hypertriglyceridemia is an atherogenic condition, the underlying mechanisms have not been well defined. We aimed to evaluate the effect of triglycerides (TGs) on leukocyte activation.
Methods: Volunteers (n=196) attended the outpatient clinic for fasting blood tests. Oral fat loading tests (n=36) were carried out to evaluate postprandial inflammation. For in vitro experiments, freshly drawn blood samples from 9 healthy volunteers were incubated with different TG emulsions: Lipoplus, Lipofundin and Intralipid and compared to LPS as positive control. Leukocyte activation markers were measured using the flow cytometer.
Results: The mean age of the total cohort was 56.7±16.5 years, 49.5% was male. Mean fasting TGs were 1.58±0.96 mmol/L. Fasting TGs were associated with monocyte CD11b (Spearman’s rho:0.29), granulocyte CD11b (r:0.30) and granulocyte CD66b (r:0.29). Significant associations were also found with HDLC (r:-0.38) and apoB (r:0.28; p<0.0005 for each) in contrast to total cholesterol and LDLC. When patients on statins were analyzed separately (n=96), most of these correlations were lost. Moreover, these patients showed a lower expression of inflammatory markers. Overall, males had a higher expression of activation markers, accompanied by higher TG levels. In the postprandial studies, the highest TGs and inflammatory markers were reached at t=4h. Positive correlations were found between the postprandial expression of CD35 (monocytes and granulocytes) and CD66b (granulocytes) with weight, BMI, SBP, DPB, waist circumference and fasting TGs (r between 0.37 and 0.65). Incubations with all TG emulsions resulted in significant increases in both CD35 and CD11b on monocytes and granulocytes, with Intralipid being the less potent activator and Lipoplus the strongest.
Conclusions: This study provides evidence for a role of TGs in leukocyte activation, most likely depending on the composition of the TG-rich particles. These inflammatory effects may be blunted by statins. Our data contribute to the understanding of the atherogenicity of TG-rich lipoproteins.
Author Disclosures: B. Burggraaf: None. M.A. de Vries: None. B. Klop: None. A. Alipour: None. A. Liem: None. P. de Man: None. G.M. van de Geijn: None. M. Castro Cabezas: None.
- © 2016 by American Heart Association, Inc.