Abstract 17064: Number of Circulating Progenitor Cells in Hypertensive Symptomatic Women With Non-obstructive CAD: a Substudy of NHLBI-Sponsored Women’s Ischemia Syndrome Evaluation-Cardiovascular Disease Cohort (WISE-CVD)
Introduction: Hypertension (HTN) is associated with inflammation and loss of structural integrity and functional impairment of the vasculature and among women carries the highest attributable risk of mortality. Circulating progenitor cells (PCs) play a key role in endothelial homoeostasis and promote vascular repair. Reductions of circulating PCs and their functional activity are associated with cardiovascular risk factors, but the relation between HTN and PCs remains unclear.
Objective: To test the hypothesis that numbers of circulating PCs are related to systolic blood pressure (SBP) in women with symptoms/signs of ischemic heart disease (IHD) without obstructive CAD.
Methods: Circulating PC counts were measured using flow cytometry for expression of CD34, CD133, CXCR4 and VEGFR2 epitopes on CD45med mononuclear cells from peripheral blood of 285 women enrolled in the WISE-CVD cohort. Correlation analyses were conducted between SBP and circulating PC numbers.
Results: The age of these women was 60±11 years (mean±SD), 34% had a history of HTN, 10% diabetes, 10% hyperlipidemia, and 7% smoking. Higher SBP correlated with lower circulating levels of CD34+ (r=-0.19, p=0.001), CD34+/CD133+ (r=-0.20, p=0.001), CD34+/CXCR4+ (r=-0.14, p=0.01), and CD34+/CXCR4+/CD133+ (r=-0.15, p=0.01). In multivariate analysis after adjusting for CAD, diabetes, hyperlipidemia, and usage of antihypertensive medications, SBP remained significantly associated with lower CD34+ (beta coefficient= -2.95, p=0.004), CD34+/CD133+ (beta coefficient= -3.48, p<0.001), and CD34+/CXCR4+/CD133+ (beta coefficient= -2.25, p=0.03). For each 10mmHg higher SBP, CD34+ percentage was 4.13 ± 1.47(Estimate ± Std Error) lower.
Conclusions: Among women with symptoms/signs of IHD without obstructive CAD, there is a significant association between SBP and reduced fraction of circulating PCs. Less circulating PCs may contribute to impaired vascular repair among women with HTN. These findings merit further investigation in other cohorts.
Author Disclosures: M. Shahrivari: Other Research Support; Significant; T32DK104721 NIDDK Training Grant to Division of Nephrology, Hypertension, and Renal Transplantation at the Univ. FL. M.S. Segal: Other Research Support; Significant; T32DK104721 NIDDK Training Grant to Division of Nephrology, Hypertension, and Renal Transplantation at the Univ. FL. C. Bairey Merz: Research Grant; Significant; NIH/NHLBI, WISE HFpEF; Gilead (RWISE); FAMRI. Consultant/Advisory Board; Modest; Gilead, Medscape (paid to institution); NIH-CASE (grant review study section). Consultant/Advisory Board; Significant; Research Triangle Institute (RTI) International. A.A. Quyyumi: None. E.K. Waller: None. C.J. Pepine: Research Grant; Significant; Capricor Inc. (ALLSTAR), inVentive (TEVA); Athersys; Vericel (ixCELL); NIH (CONCERT HF HL087318; CCTRN-2 HL087366; CTSI TR001427).
- © 2016 by American Heart Association, Inc.