Abstract 16717: Customizable High-density Microelectrode Arrays for Murine Cardiac Electrophysiology
Introduction: Mouse models offer robust approaches to study cardiac physiology and pathophysiology in part due to the ability to manipulate their genome. Traditional methods for recording murine cardiac electrophysiologic (EP) data involve electrocardiograms and small catheters in vivo which provide limited spatial resolution, or optical mapping ex vivo which requires electromechanical uncoupling.
Objective: To develop high-density microelectrode arrays for high-fidelity, high-resolution recording of murine cardiac EP data.
Methods & Results: We have developed high-density microelectrode arrays for recording unipolar electrocardiograms and pacing from the epicardial surface of the murine heart (Fig. 1A). These arrays are composed of a thin-film polymer with a grid of addressable electrode sites. The highly flexible polymer substrate maintains conformal contact with the heart as it beats. The design space for the arrays include grid dimensions from approximately 200 μm to 8 cm and 16 to 512 electrodes, with each electrode having the capacity to record and stimulate. The array in Fig. 1A has a 1.3 x 2.5 mm footprint with 32 electrodes spaced 300 μm apart. It is connected to a digital headstage through a flexible ribbon cable and then to a data acquisition system. Using this approach, we demonstrate that we can record unipolar electrograms and pace from the ventricular epicardium of a murine heart (Fig. 1B). These data can be used to calculate activation times, repolarization times and activation-recovery intervals, a surrogate for local action potential duration, as well as corresponding propagation maps (Fig. 1C).
Conclusion: We have developed customizable high-density microelectrode arrays for high-fidelity, high-resolution recording of cardiac EP data from intact murine hearts, in both in vivo and ex vivo settings. These arrays can be scaled in size and electrode density to be used in larger animal models as well as humans.
Author Disclosures: P.S. Rajendran: None. O.A. Ajijola: None. R. Vetter: None. A. Snellings: None. J.D. Tompkins: None. A. Deb: None. D.R. Kipke: None. K. Shivkumar: None. J.L. Ardell: None.
- © 2016 by American Heart Association, Inc.