Abstract 16173: Changes in Extracellular Circulating MicroRNAs Are Associated With a Weight Loss Intervention in Filipinos
Introduction: A moderate amount of weight loss through lifestyle modification is an effective treatment for prevention of type 2 diabetes (T2D) for some individuals, however others remain at risk. Filipinos have one of the highest prevalence of T2D. MicroRNAs (miRs) are circulating biomarkers associated with risk factors for T2D and may also be linked to lifestyle modification responses.
Hypothesis: Expression of circulating miRs will change in response to a 3-month tailored weight loss intervention.
Methods: Eight Filipino participants (38 ± 6 years, 50% men) who completed a tailored weight loss intervention were analyzed. Blood was collected at enrollment and after 3-months of the intervention. The Firefly Bioworks Multiplex Circulating MicroRNA Assay was used to quantify 337 miRs. Fold changes and student’s t-test were calculated.
Results: After 3-months of the intervention, the mean weight loss was 9 ± 4 pounds. Total cholesterol (-14 ± 31mg/dL), LDL-c (-5 ± 10mg/dL), triglycerides (-55 ± 186mg/dL), fasting blood glucose (-2 ± 3mg/dL) decreased and HDL-c (1 ± 6mg/dL) and hemoglobin A1C (0.8 ± 2.2) increased. Four miRs were increased (i.e., miR-652, miR-24, p<0.05; miR-326, miR-425, p<0.1) and nine miRs were decreased (i.e., miR-140, miR-532, miR-20b, p<0.05; miR-106b, miR-363, miR-92a, let-7c, miR-93, miR-486; p<0.1) following the 3-month intervention. There were moderate correlations between baseline fasting blood glucose and miR-326 (r2=0.3), let-7c (r2=0.4), and miR-101 (r2=0.4).
Conclusions: These data represent the first known study of changes in extracellular miRs over time in response to a weight loss intervention in high risk Filipinos. We validated previously seen changes in miRs (e.g., miR-20b, miR-miR-486) and identified novel targets that warrant further investigation as biomarkers for T2D risk, responses to interventions, and mechanistic pathways.
Author Disclosures: E. Flowers: None. M. Bender: None. Y. Fukuoka: None.
- © 2016 by American Heart Association, Inc.