Abstract 16008: An Association Between Short Telomere Length and Major Cardiovascular Events is Influenced by the Proximity of Telomere Measurement to Event Occurrence
Background: Short telomere length (TL) is associated with cardiovascular (CV) events. However, disagreement exists as to the physiological association between TL and CV events. Short TL may indicate pathology predisposing to an event as would be seen in a prospective study, or may occur subsequent to an event which would only be observed retrospectively.
Objective: To determine whether prevalent (P) and incident (I) CV events differ with respect to association with TL.
Methods: Peripheral blood TL was obtained from consenting patients (pts) undergoing angiography (n=3576). TL was determined by multiplex quantitative PCR that amplified both the telomere repeat and the single copy gene (albumin) used to normalize the result for DNA input. The quantitative PCR reactions were performed in triplicate and signal generated by the incorporation of the monochrome dye (SYBR Green I) Signal was measured by PCR (Bio-Rad CFX384 Detection System).TL is reported as t/s, the normalized ratio of telomere to single gene measurements; t/s was age stratified for group comparisons. Clinical events (MI and CVA) were determined by ICD9 codes; pts were followed for a mean of 2482 days (max 6342) from the time of blood draw.
Results: In all 1071 (29.9%) subjects experienced 1293 (602 P and 766 I) events. For P events, mean t/s was significantly shorter for pts with an event compared to those with no events (0.96 vs 1.01, respectively, p<0.03). For I events, t/s varied by the time to event (TTE): t/s for TTE quartile (Q) 1 (< 1200 days) was significantly shorter than for Q4 (> 3487 days), 0.92 vs 1.03, respectively; p<0.0009 (see figure). Q1 t/s was also shorter than t/s for pts with no events (0.88 vs 1.01, respectively; p<0.004)
Conclusion: Short t/s is significantly associated with prior events; the relationship with incident events is dependent on the time between t/s measurement and the event. These results are consistent with short TL etiologically associated with CV events, but further study is necessary.
Author Disclosures: J.F. Carlquist: None. S. Knight: None. R. Cawthon: None. B.D. Horne: None. J.B. Muhlestein: None. J.L. Anderson: None.
- © 2016 by American Heart Association, Inc.