Abstract 15421: Effect of Weight Loss Intervention Program on Cardiac Function, Endothelium Progenitor Cells and Microparticles in Mildly Obese Asians
Introduction: The effect of weight lost through lifestyle intervention on cardiac function are not well elucidated, especially in Asians where body mass index (BMI) cut-offs are lower than in the West. The balance between endothelium progenitor cells (EPCs) (endothelium repair) and microparticles (EMPs) (endothelium damage) has also not been explored in the effects of weight loss in Asians.
Methods: Healthy subjects (38±5 years; n=55; BMI=27.1±1.7 kg/m2) were recruited into 4 month weight loss intervention program. Comprehensive transthoracic echocardiography with speckle tracking imaging were performed. Subjects were encouraged to perform physical activity (>10,000 steps of walking per day monitored by pedometer), consume meal replacement and attend dietitian visits. Enumeration of EPCs and EMPs were performed using flow cytometry. Migration and tubule formation assays were also conducted to determine EPC function.
Results: With a mean daily intake of 1300 calories over 4 months, there was significant 9.4% weight loss, reduction in BMI (27.1±1.7 to 24.8±1.7 kg/m2), waist circumference (91±7 to 82±8 cm), neck circumference (33±3 to 31±3 cm), systolic blood pressure (121±12 to 115±12 mmHg) and diastolic blood pressure (68±10 to 64±8 mmHg) (all Ps<0.001). Furthermore, EPC number (0.35±0.08 to 0.45±0.05%), migrated cell (7.5±2.1 to 8.9±1.7), tubule formation (8.5±2.8 to 10.1±2.9 mm2) and EMPs (102±14 to 85±6/μL plasma) improved after dietary intervention (P<0.01). Cardiac output (4.33±0.78 to 4.03±0.87 L/min) was also reduced, associated with lower heart rate (68±9 to 63±13 bpm) (P<0.05). There was decreased left ventricular filling pressure, with septal E/E’ (6.6±2.0 to 5.9±1.4) and lateral E/E’ (8.4±1.7 to 7.9±1.9) (P<0.01). There was significant increase in global longitudinal strain (-18.2±2.6 to -19.3±2.8 %) (P=0.03), which may represent improvement in subclinical left ventricular function.
Conclusions: In healthy mildly obese Asians, weight lost through lifestyle intervention, have successfully restored the balance between vascular endothelial repair and damage. There was improvement in global longitudinal strain, which may serve as an important predictor of subclinical left ventricular function in obesity.
Author Disclosures: P.S. Lee: None. F. Naseer: None. S. Lim: None. E.Y. Khoo: None. T. Yeo: None. A. Richards: None. K. Poh: None.
- © 2016 by American Heart Association, Inc.