Abstract 15321: Increased Expression of Mircorna-21 Contributes to Reduced Microvascular Function in Young Adult Regular Binge Drinkers
Departments of Medicine1, Biobehavioral Health Science2, Physical Therapy3, Kinesiology and Nutrition4, The University of Illinois at Chicago
Binge drinking (BD) is one of the biggest health dangers confronting college students and compared to previous generations, the pervasiveness, regularity, and intensity (i.e., 6-7 drinks) of BD may place today’s youth at greater risk for more profound rates of alcohol-induced harm and premature disease. Micro RNAs are a class of molecules involved in a large number of cellular functions and changes in miRNAs may play a role in gene-environment interactions related to cardiovascular (CV) disease. The aim of this study was to determine if microvascular function is reduced in BD and if increases of microRNA-21 (miR-21) contribute to this dysfunction.
Methods: Gluteal subcutaneous adipose biopsies were obtained from young adults (mean age 23 yrs, n=5 BD and n=5 abstainers (A)). Resistance arterioles (RAs) were isolated and vasoreactivity responses to flow (10-100 cmH2O pressure gradients) and to acetylcholine (ACh, 10-9-10-4 M) were determined in the presence and absence of miR-21 inhibitor (50nM) or the scrambled negative control (50nM). Real time quantitative PCR (RT-qPCR) was used to measure endogenous miR-21 mRNA level.
Results: Mean duration of BD was 2.4 years and mean AUDIT scores were 11±7 in BD compared to 0.8±0.8 in A (p < .03). Responses of RAs to flow and ACh were reduced in the BD group compared to A. Overnight incubation with an anti-miR-21 peptide, but not with a negative control, restored both ACh- and flow-induced vasodilation in BDs while the both agents have no effects in A. Finally, miR-21 mRNA expression was increased 4.5-fold in adipose tissues from BD compared to the A group.
Conclusion: These data indicate BD is associated with microvascular dysfunction and that increased microRNA-21 upregulated mRNA in the vascular wall may contribute to reduced flow and ACh dilation in binge drinkers (NIH, R01HL095701-SAP, R21AA015578-MRP).
Author Disclosures: J. Bian: None. A.M. Mahmoud: None. K.U. Kotlo: None. M.R. Piano: None. S.A. Phillips: None.
- © 2016 by American Heart Association, Inc.