Abstract 14693: Sodium Hydrosulfide in the Setting of Cardioplegia and Cardiopulmonary Bypass in the Rat Model Reduces Ischemia-Reperfusion Injury
Introduction: Sodium hydrosulfide (NaHS) has been shown to be a promising cardioprotective agent against ischemia-reperfusion injury, but its use as a component of cardioplegia in cardiopulmonary bypass (CPB) is untested. We investigated the potential cardioprotective effects of NaHS added to cardioplegia solution, in rats in cardiac arrest on CPB.
Methods: Adult male Wistar rats (n=16) were placed on CPB and underwent 30 minutes of aortic cross-clamp (AoX)/ischemia, followed by 1 hour of reperfusion. Rats in the treatment group (n=8) received 40uM of NaHS in the cardioplegia solution, rats in the control group (n=8) did not. Hemodynamic parameters were measured intraoperatively and blood samples were collected at baseline, before and after AoX, and after weaning off CPB. Hearts were harvested at the end of the procedure.
Results: The dP/dT max, indicative of systolic function, was better preserved in treatment groups post-CPB (32.7% decrease vs. 56.8% decrease from baseline) (p<0.01). Similar trends were observed for dP/dT min post-CPB (38.6% decrease in treatment vs. 59.2% decrease in control group) (p<0.05). The increase in relaxation time (tau) between treatment (196%) and control (163%) groups, as well as the decrease in heart rate between treatment (30.3%) and control (39.0%) groups was not significant. Serum Troponin I levels were lower in the treatment group immediately after release of AoX (p<0.05), but their levels were comparable post-CPB (Figure 1E). Myocardial ATP content was also lower (p<0.01) in the treatment group post-CPB indicating better ability of myocardium to utilise ATP.
Conclusions: NaHS in cardioplegia solution significantly improves systolic function, diastolic function and ATP utilisation (which in turn results in improved hemodynamic parameters). It is superior to plain cardioplegia in reducing myocardial ischemia-reperfusion injury in the rat CPB and cardiac arrest model.
Author Disclosures: C. Ong: None. Y. Ong: None. D. Vu: None. M. Oo: None. A. Rufaihah: None. E. Ocampo: None. T. Kofidis: None.
- © 2016 by American Heart Association, Inc.