Abstract 14384: Supplementation With Epa and With Dha Induces Similar Inflammatory Gene Expression Profiles in Human Whole Blood Cells: The Compared Study
Introduction: Low-grade inflammation is recognized as a key etiological factor in the development of CVD. Whether DHA and EPA have similar anti-inflammatory effects remains unclear, as most of the available studies have used a mixture of the two fatty acids in various forms and proportions.
Hypothesis: We hypothesized that DHA is more potent than EPA in activating inflammatory gene pathways.
Methods: Blood samples were obtained from a subset of 44 subjects originating from the double-blind controlled crossover study, where 154 subjects with abdominal obesity and low-grade inflammation were randomized to three 10-wk treatment phases: 1- EPA (2.7g/d); 2- DHA (2.7g/d); 3- corn oil (control), each separated by 9-wk washouts. Supplements were provided as 1g capsules for a total of 3g/d. Expression of pro- (CD14; TNFA) and anti- (PPARA; TRAF3) inflammatory genes was measured on whole blood cells by RT-qPCR after each treatment.
Results: EPA vs. control enhanced TRAF3 and PPARA expression (P<.01) and lowered expression of CD14 (P<.05). DHA vs. control increased expression of PPARA and TNFA (P<.05). However, no significant difference was observed between EPA and DHA in the expression of these genes. Variations vs. control in the gene expression in response to DHA and EPA were strongly correlated for PPARA (R2=52.6%, P<.0001) and TRAF3 (R2=43.3%, P<.0001), less so for TNFA (R2=21.0%, P<.005) and not correlated for CD14 (R2=2.6%, P=.30). Finally, the DHA-induced reduction in plasma CRP vs. control (-18±10%, P=.19) was consistent with concurrent variation in the expression of PPARA (R2=19.6%, P<.01) and TRAF3 (R2=16,6%, P<.01). No such association was observed in response to EPA.
Conclusions: High-dose supplementation with EPA and DHA has similar effects on the expression of many inflammatory genes in whole blood cells of men and women at cardiometabolic risk. Changes vs. control in anti-inflammatory gene expression after EPA and DHA supplementations appeared to be more consistent than changes in the expression of pro-inflammatory genes. Finally, the CRP reduction with DHA vs. control seems to be concomitant to up-regulated anti-inflammatory gene expression rather than down-regulation of pro-inflammatory genes.
Author Disclosures: C. Vors: Research Grant; Significant; FRM (Fondation pour la Recherche Médicale). J. Allaire: Research Grant; Significant; CIHR, FRQS (Fonds de recherche du Québec Santé). J. Marin: None. M. Lépine: None. A. Charest: None. A. Tchernof: Research Grant; Significant; Investigator-initiated support grant from Johnson & Johnson Medical Companies. P. Couture: None. B. Lamarche: Research Grant; Significant; CIHR. Other Research Support; Significant; Atrium Innovations.
- © 2016 by American Heart Association, Inc.