Abstract 14088: Advanced Glycation Endproducts (AGEs) Fluorescence as a Biomarker for Risk of Diabetes Patients
Introduction: Advanced glycation endproducts (AGEs) are non-enzimatic glycation of proteins, lipids or nucleic acids formed during normal life. However, the accumulation of accelerated AGEs can occur due to increased oxidative and glycemic stress. This has led to a cross-linking of proteins, mainly collagen from skin. In this context, fluorescent AGEs, such as pentosidine, have been measured by optical techniques. Fluorescence spectroscopy may provide opportunities for diagnosis due to low-cost screening, portability and the possibility of a real-time response using a noninvasive and nondestructive investigative method. Biological tissues contain chromophores that absorb light, as well as fluorophores that absorb and reemit light to help in the initial screening of several diseases, such as diabetes.
Hypothesis: Fluorescence spectroscopy of skin can identify AGEs in diabetes patients.
Methods: Ninety four women and men aged 20 to 80 years with or without type 1 or 2 diabetes were evaluated. The fluorescence spectrometer was used to illuminate a skin surface with a peak excitation of 370 nm (ultraviolet A). The right forearm was posited on the device to measure the skin autofluorescence. Three measurements were performed to obtain an average of score. Body mass index (BMI), waist and hip circumferences, body fat percentual, heart rate (HR) and blood pressure (BP) were measured.
Results: There were prevalence of the 15% for hypertension, 20% for dyslipidemia, 30% for sedentary lifestyle, 53% for social alcohol intake and 100% for non-smokers among people investigated. The AGEs fluorescence score was significantly higher in diabetic patients compared with healthy subjects (3.3±1.0 vs 1.8±0.5, p<0.05). AGEs score was significantly correlated to body fat (r=0.48, p=0.0001), waist (r=0.35, p=0.001) and systolic BP (r=0.37, p=000.1).
Conclusions: Highly accelerated AGEs are present in diabetic patients, which are considered one of the important pathogenic mechanisms resulting in organ damage and severity of diabetes-related complications. Therefore, fluorescent AGEs in the skin may be a convenient way for noninvasive screening of diabetes and risk of related complications associated with AGEs accumulation.
Author Disclosures: F.R. Paolillo: None. A. Borghi-Silva: None. V.S. Mattos: None. G. Arthuzo: None. J.C. de Castro Neto: None. V.S. Bagnato: None.
- © 2016 by American Heart Association, Inc.