Abstract 13898: Simvastatin Inhibited Myocardial Apoptosis Undergoing Noncoronary Artery Cardiac Surgery
Introduction: We previously demonstrated that simvastatin was cardioprotective in patients undergoing noncoronary artery cardiac surgery. However, the mechanisms by which simvastatin protected myocardium were not fully understood.
Hypothesis: Simvastatin protects myocardium is related to the inhibition of apoptosis
Methods: 35 patients undergoing noncoronary cardiac surgery such as valvular surgery, congenital heart diseases surgery, 19 from simvastatin group and 16 from untreated control group, were selected from a previous clinical trial. Simvastatin (20mg) was administered preoperatively for 5-7 days. Myocardial tissues biopsies were taken in the right atrial appendage before cross-clamping the aorta and heart beating 10 minutes after removing the aorta cross-clamp. The apoptosis in myocardium was detected by transmission electron microscope and TUNEL staining. The expression of Bcl-2 and Bak were detected by Western blot.
Results: In the untreated controls, electron microscopic analyses revealed evidence for ischemic injury in cardiomyocytes characterized by swollen mitochondria with vacuoles, and shortened and disrupted cristae. Simvastatin significantly reduced these changes compared to the control group. TUNEL staining showed that myocardial apoptosis in simvastatin group was much less than that in the control group. Simvastatin significantly upregulated the expression of Bcl-2 and downregulated the expression of Bak after surgery.
Conclusions: The mechanisms by which simvastatin protected myocardium undergoing noncoronary cardiac surgery is, at least in part, related to the inhibition of apoptosis, which may be related to the preservation of mitochondrial structure and function.
Author Disclosures: J. Ou: Research Grant; Significant; This study was supported by the National Natural Science Foundation of China (81170271, 81370370 and Distinguished Young Scholar 81325001), the Changjiang Scholars Program from Ministry of Education o. L. Zhou: None. X. Liu: None. Z. Wang: None. Z. Ou: None. Y. Xu: None. Z. Wang: None.
- © 2016 by American Heart Association, Inc.