Abstract 12037: Metabolite Profiles Associated With Variation in Visceral Adiposity: The Multi-Ethnic Study of Atherosclerosis
Introduction: Understanding the association between serum metabolites and variation in visceral adipose tissue (VAT) content could provide novel insights into the pathogenesis and prevention of obesity and obesity-associated diseases.
Methods: We analyzed data from participants in the Multi-Ethnic Study of Atherosclerosis who underwent untargeted metabolomics profiling of blood serum collected between 2000-2002 by proton nuclear magnetic resonance (1H NMR) spectroscopy on a Bruker DRX600 spectrometer at 600 MHz using a standard 1-D spectrum and subsequent assessment of VAT area (cm2) between 2002-2005 by computed tomography. Multivariable linear regression was used to evaluate the associations between NMR metabolic features and VAT area. Metabolites were identified with spiking and peak connectivity and with spectral library verification. A false discovery rate threshold of 1% was applied for statistical significance.
Results: 1,102 participants were included with median age 63 years; 52% male; 40% white; median BMI 27 kg/m2. 30,590 metabolomic spectral features were evaluated. After multivariable adjustment for age, sex, race, data acquisition phase (batch 1 or 2), socioeconomic status score, smoking, physical activity, and body mass index, 2,113 spectral variables representing 14 distinct metabolites were significantly associated with VAT area (Figure). After additional adjustment for fasting plasma glucose or diabetes status, all metabolites except glycerol remained statistically significant.
Conclusions: Using an untargeted metabolomics approach, we found a distinct BMI and glucose independent metabolite profile associated with excess visceral adiposity. These findings provide insight into the pathophysiologic and metabolic mechanisms associated with variation in VAT burden. Further identification of unknown metabolites and external validation are needed.
Author Disclosures: I.J. Neeland: None. C. Ayers: None. I. Tzoulaki: None. I. Karaman: None. C. Boulange: None. D. Vaidya: None. N. Punjabi: None. M. Allison: None. D. Herrington: None. P. Greenland: None.
- © 2016 by American Heart Association, Inc.