Abstract 11983: Delayed Inhalation of Hydrogen Mitigates Neurological Dysfunction in a Porcine Model of Cardiac Arrest and Cardiopulmonary Resuscitation
Introduction: Immediate inhalation of hydrogen (H2) has been demonstrated to improve post-resuscitation (PR) neurological dysfunction. However, early applying inhaled H2 is not practical especially in out of hospital cardiac arrest because of the safety issue. In the present study, we investigated the effects of delayed treatments with H2 on post resuscitation neurological function in a porcine model of prolonged cardiac arrest (CA).
Hypothesis: Delayed inhalation of H2 mitigates neurological dysfunction after resuscitation in a CA porcine model.
Methods: Eighteen male domestic pigs weighing 39±2 kg were utilized. Ventricular fibrillation (VF) was induced electrically and CPR was initiated after 10 mins of untreated VF. All the animals were resuscitated successfully and randomized into two groups immediately following resuscitation: delayed inhalation of H2 (DH group, N=9) or inhalation of room air (C group, N=9). Animals in the DH group were ventilated with 2% H2/21% oxygen from 2h post resuscitation (PR) to PR 4h. Serum levels of S100B and neuron-specific enolase (NSE) were measured by ELISA at baseline and PR 360 min. The neurological deficit score (NDS) and survival were evaluated daily for a total of 72 hours.
Results: Better survival duration and NDS at 24h, 48h and 72h were observed in the DH group animals compared with those of the C group (Table). The serum levels of S100B and NSE were significantly lower in the DH group when compared with the C group (Figure).
Conclusion: Delayed treatment of H2 mitigates neurological injury and improves neurological dysfunction after resuscitation.
Author Disclosures: Z. Yang: None. T. Yu: None. C. Wen: None. X. Zhai: None. M. Peberdy: None. J. Ornato: None. W. Tang: None.
- © 2016 by American Heart Association, Inc.