Abstract P278: Metabolomic Profiles Associated with Dietary Patterns in Women
Background: Dietary patterns have a complex impact on metabolism with certain foods increasing specific metabolite levels. Metabolomic profiling, an emerging technology, may offer insights into the biological mechanisms linking the Western dietary pattern (WD) with cardiovascular disease and cancer.
Methods: Metabolomic profiling of 371 metabolites by tandem quadrapole liquid chromatography/ mass spectroscopy was available on 1153 healthy, control subjects in the Women’s Health Initiative. Metabolites were log-transformed and standardized and each considered individually in statistical models. Using factor analyses, we analyzed dietary data and identified two major dietary patterns: WD (high in meat, poultry, fish, solid fat, oils, cheese, processed meats, refined grains, and added sugars) and Prudent dietary pattern (PD) (high in fruits and vegetables). Linear regression models were created with the dietary pattern scores (WD or PD) as a continuous variable for the primary exposure and each metabolite as the outcome. Models were adjusted for matching factors in the primary study (race, time period, hysterectomy status), as well as for body mass index and medications (antihypertensives, anti-diabetic drugs, aspirin and statins). Significant results were defined as FDR adjusted p<0.05.
Results: Median age was 68 years (interquartile range 62-72). After multivariable adjustment, WD was inversely associated with 2 metabolites: eicosapentaenoic acid (EPA) and docosahexaenoic acid DHA (FDR p=0.01); these were positively associated with the PD, (FDR p<0.0001). PD was significantly associated with 85 metabolites (mostly lipids) including 4 phosphatidylcholines (FDR p<0.0001); among significant inversely associated metabolites were 11 acylcarnitines (e.g.C4-OH-carnitine, FDR p <0.0002), 2 polyunsaturated fatty acids (docosatrienoic acid, FDR p=0.0004 and myristoleic acid, FDR p=0.049), and 12 plasmalogens (e.g. C36.2, C36.3, C36.4 phosphatidylethanolamines,FDR p<0.0001).
Conclusions: PD is associated with multiple metabolites involved in mitochondrial fatty acid oxidation, as well as several associated with insulin resistance. EPA and DHA levels were lower in those with WD and higher among those with PD. These metabolites may be involved in how dietary patterns impact chronic disease.
Author Disclosures: P.D. Chandler: B. Research Grant; Significant; American Cancer Society, Career Development Award 127524-MRSG-15-012-01-CNE. R. Balasubramanian: None. N. Paynter: None. F. Giulianini: None. L.G. Snetselaar: None. S. Liu: None. C. Eaton: None. D. Tobias: None. J.E. Manson: None. E. Giovannucci: None. C. Clish: None. K.E. Rexrode: None.
- © 2016 by American Heart Association, Inc.