Abstract P147: Dietary Capsaicinoids May Enhance Exercise by Effective Utilization of Lipids When Combined with Treadmill Running in a Rat Model
Objective: Capsaicinoids (CAPs) are the major pungent, naturally occurring active compounds in capsicum fruits such as hot chili peppers. We recently demonstrated that CAPs inhibits pancreatic lipase and down regulates C/EBPα and PPARγ. Therefore, we hypothesized that CAPS may enhance exercise by effective utilization of lipids in rats. In this study, we observed changes in protein expressions levels of NFkB, IL-10, Nrf2, pAMPK and HO-1 in rats fed a diet containing CAPs.
Methods: Eight week old male Wistar rats were treated in four treatment groups (i) control [no Ex, Group I (CTL)] (ii) CTL + CAPs [no Ex, 0.2 mg capsaicinoids from 10 mg/kg BW/day Capsimax®, Group II] (iii) CTL + Ex [Group III] and (iv) CTL + Ex+ CAPs [0.2 mg capsaicinoids from 10 mg/kg BW/day Capsimax®] for 8 wks. The Ex protocols were performed on a motor-driven rodent treadmill (TMR). All animal procedures were approved by the Animal Experimentation Ethics Committee of Inonu University (Malatya, Turkey; 2014/A-55). Blood analysis for triglycerides (TG) and cholesterol (CHOL), muscle analysis for lactate, superoxide dismutase (SOD) and glutathione peroxidase (GPx) and malonaldehyde (MDA a marker of oxidative stress [OS]) were estimated. Phosphorylated AMP-activated protein kinase (pAMPK), Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), heme oxygenase-1 (HO1), nuclear factor (NF-κB) and Interleukin-10 (IL-10) proteins in the gastrocnemius muscle was evaluated by Western blotting.
Results: As shown in Table, Group IV significantly increased running performance and exhaustion time and a significant decrease in TG and CHOL was observed compared with other treatments. A significant decrease in lactate and MDA and increase in SOD and GPx were observed in Group IV. An increase in protein levels of pAMPK, Nrf2, and HO1 and a decrease in NF-κB and IL-10 levels were observed in Group IV.
Discussion: These results suggest CAPs with Ex may enhance the effect of exercise by effective utilization of lipids by increasing pAMPK and Nrf2.
Author Disclosures: V. Juturu: A. Employment; Significant; Employee. K. Sahin: B. Research Grant; Modest; Grant. C. Orhan: None. R. Pala: None. M. Tuzcu: None. N. Sahin: None.
- © 2016 by American Heart Association, Inc.