Abstract 18723: In vivo Tissue Engineered Small Diamiter Vascular Graft “Biotube”; Application to Off-pump CABG in the Porcine Model
Objective: In spite of the great labor and fund for the development of the small diameter vascular prostheses applicable to the coronary arterial bypass grafting (CABG), the only available grafts are still autologous arteries and veins. Aiming for the application to CABG and below knee distal arterial bypass grafting, we have developed autologous small diameter grafts (ID; 1.5 ~ 5 mm) “Biotubes” constructed in the recipient bodies. In AHA 2006, we have already reported that "BIOTUBEs" could be ideal small-caliber vascular grafts in rabbit model. In this study, we evaluate the feasibility of Biotubes to the off-pump CABG using an acute xenogeneic porcine model.
Materials & Methods: Silicon rod molds (diameter; 3 mm, length; 15 cm) were embedded in the subcutaneous pouches of beagle dogs for 4 weeks to obtain Biotubes. They were xenogenically implanted to swine using conventional off-pump CABG technique (n=5). Top-end anastomosis of each Biotube was placed on innominate artery using 8-0 polypropylene sutures. Its distal end was anastomosed to the side of left anterior descending coronary artery (LAD) with 8-0 sutures using cardiac stabilizer.
Results: The obtained Biotubes had very thin wall (less than 0.1 mm) mainly consisting of fibroblasts and collagen fibers as previously reported. However, anastomoses of Biotubes to native arteries were possible even under off-pump surgery with a little difficulty. Postoperative angiography and doppler flow meter confirmed their patency and durability without rupturing or aneurysm formation.
Conclusion: Application of Biotubes to the off-pump CABG was technically feasible in an acute swine model. Long-term animal CABG implantation study using auto-grafts is planed in the next stage for clinical use.
Author Disclosures: T. Watanabe: None. K. Kanda: None. M. Yamanami: None. H. Yaku: None. Y. Nakayama: None.
- © 2015 by American Heart Association, Inc.