Abstract 16651: Development of a Novel Cardiac Arrest Model in Aged Mice
Introduction: Cardiac arrest (CA) occurs commonly in daily life, especially for elderly patients. However, most studies in CA and cardiopulmonary resuscitation (CPR) were performed in young animals. A relevant CA/CPR experimental model in aged animals is needed for better understanding the mechanisms of this disease and seeking advanced therapeutic approaches. In this study, we aimed to develop a novel mouse model that can be used to investigate CA/CPR in aged mice.
Hypothesis: The aged mice subjected to CA/CPR would be resuscitated.
Methods: Male C57Bl6 mice were subject to 8 min of KCl induced cardiac arrest and then resuscitated by epinephrine infusion and chest compression. The use of KCl was minimized by withdrawal of blood from jugular vein for better resuscitation and recovery. 22 months old mice were performed and 8-10 weeks old mice served as reference (n=10 per group). All mice had withdrawal of 0.5 ml of blood followed by infusing 30μl 0.5 M KCl to induce flat EKG. Blood was infused back at 3 min after onset of cardiac arrest. Epinephrine (32μL/mL) was slowly infused 30 seconds before resuscitation until regular EKG appeared. Resuscitation time and survival rate were recorded. Spontaneous activity was measured in smart cage at pre-injury and days 1, 2, and 3 post-injury. Neurological score (9 points ) was measured at day 3 after CA/CPR.
Results: All mice were successfully resuscitated and survived for 24 hours. Young mice restored spontaneous heart activity within 1 min and however, most of aged mice took more than 1 min (p=0.014). Aged mice had a significantly decline of spontaneous activity including travel time and travel distance (travel time p=0.02 compared to young mice group, Fig 1). 3 days of survival rate was 40% in aged mice and 90% in young mice (p=0.03, Fig 2). Aged mice also had a worse neurological score at day 3 post-injury (p=0.026).
Conclusions: This modified procedure ensured all mice resuscitated and survived for 24 hours. Declined spontaneous activity and neurological function represent the success of this disease model. Our on-going experiment is to define the age related stress responses and SUMO conjugation using this novel mouse model and seek new targets for treatment of this injury.
Author Disclosures: H. Sheng: None. H. Liu: None. B. Xu: None. Z. Yu: None. W. Paschen: None. D.S. Warner: None. W. Yang: None.
- © 2015 by American Heart Association, Inc.