Abstract 15833: Circulating MicroRNAs Provide Insight Into Potential Molecular Links Between Hypertensive Disorders of Pregnancy and Cardiovascular Disease
Introduction: Hypertensive disorders of pregnancy, such as preeclampsia and gestational hypertension, are strong sex-specific risk factors for cardiovascular disease (CVD). The molecular determinants underlying this risk have not been clearly defined, but potentially involve sustained vascular damage and/or dysfunction. This may be reflected by alterations in the levels of specific circulating microRNA (miRNA).
Study Objective: To identify miRNAs that circulate at different levels in women diagnosed with acute coronary syndrome (ACS), with and without a prior history of preeclampsia or gestational hypertension.
Methods: The present study is a derivation cohort of women with a diagnosis of premature (age < 55 yrs) ACS, divided into three groups based on a prior history of gestational hypertension, preeclampsia, or normotensive pregnancy (n=11-13/group). The three groups were closely matched on potential confounding variables including age, chronic hypertension, diabetes, smoking status and type of ACS. Total RNA was extracted from citrate plasma of each participant, and the relative levels of 372 miRNAs were measured by high-density PCR array. Measurements and analyses were performed blinded to exposure status.
Results: The circulating levels of 16 miRNAs were significantly (p<0.05) altered in the preeclampsia versus normotensive pregnancy groups (from -2.8 fold down to +2.0 fold change), and 32 miRNAs were significantly altered between the gestational hypertension and normotensive pregnancy groups (from -2.8 to +2.7 fold change). These miRNAs showed little to no overlap with previously published reports of circulating miRNAs that were altered acutely at the time of preeclamptic pregnancy or ACS event. Several priority candidates have previously been functionally annotated, including miRNAs linked to angiogenesis (miR-126-3p), inflammation (miR-146a-5p), and cholesterol metabolism (miR-122-5p).
Conclusions: Circulating levels of multiple miRNAs were altered between female ACS patients with and without prior hypertensive disorders of pregnancy. These miRNAs provide novel insight into the potential molecular pathways that may contribute to vascular dysfunction and risk of CVD.
Author Disclosures: N. Dayan: None. K. Schlosser: None. D.J. Stewart: None. M. Okano: None. S. Grand’Maison: None. L. Pilote: None.
- © 2015 by American Heart Association, Inc.