Abstract 15623: Transplantation of Adipose-derived Stem Cells Sheet for Accelerated Neovascularization and Engraftment in Acute Myocardial Infarction Rats
Background: Transplantation of adipose-derived stem cell (ADSCs) has been suggested to improve cardiac function after acute myocardial infarction (AMI). Poor retention rate and differentiation ability of the implanted ADSCs limited their therapeutic potential. This study investigated the application of cell sheet technology for ADSC to improve engraftment and functional recovery in rat AMI model.
Methods: Mouse ADSC (mADSC) sheets were successfully obtained by using the thermo-responsive dishes. Three groups of sham (n=10), suspended mADSC myocardial injection (n=10) and mADSC sheet transplantation (n=10) were compared in rat AMI model of left anterior descending coronary artery ligation. Final analyses were performed at day 28 after cell implantation.
Results: In rat AMI model, mADSC sheet showed higher cellular engraftment in the infarcted heart compared to suspended mADSCs and sham control (signal unit; 227.3±18.3, 2.0±2.0 and 0; p<0.05). Cytokine analysis of the cell-implanted myocardial tissues showed that higher expression of IGF-1, bFGF, TGF-β1, and EGF in mADSC sheet compared to suspended mADSCs and sham control (p<0.05). Microvascular density of the peri-infarct zone was also higher in mADSC sheet compared to suspended mADSCs and sham. Interestingly, the transplanted cell-derived microvessels determined by mouse specific anti-vWF antibody were detected only in mADSC sheet. Echocardiography showed the significant improvement of ejection fraction (EF) in both suspended mADSCs and mADSC sheet compared to sham control (ΔEF; 15.60±0.44%, 15.24±0.62% and 3.53±%0.18; p<0.05).
Conclusions: Although the functional recovery was comparable between ADSC sheet transplantation and suspended ADSC injection, ADSC sheet transplantation improved the cellular engraftment, paracrine effect of growth factor and cytokine secretion, and in-vivo neovascularization compared to suspended ADSC injection.
Author Disclosures: J. Kim: None. H. Joo: None. M. Kim: None. S. Choi: None. C. Park: None. J. Lee: None. S. Hong: None. D. Lim: None.
- © 2015 by American Heart Association, Inc.