Abstract 15099: Suppression of Autophagic Flux by Sequestration of p62 Promotes RIP1-mediated Necroptosis in Cardiomyocytes
Purpose: Necroptosis, a programmed form of necrosis, has been implicated in various pathological conditions including heart failure. Here we examined whether and how receptor-interacting protein kinase (RIP) 1-dependent necroptosis is functionally linked with autophagy in cardiomyocytes.
Methods and results: In H9c2 cells, the necroptotic pathway was activated by TNF-α (TNF, 50 ng/ml) alone or by co-incubation of TNF with 20 mM z-VAD-fmk (zVAD), a pan-caspase inhibitor. TNF increased LDH activity in the culture medium from 26.6±4.3% of total LDH activity in the vehicle-treated control to 39.1±4.8%, suggesting induction of necrosis. Addition of zVAD to TNF (TNF/zVAD) further increased the LDH activity to 70.6±2.7%. The augmented cell death by TNF/zVAD was completely reversed by necrostatin-1 (39.4±4.0%), a RIP1 inhibitor, and was attenuated by rapamycin (10 nM), a promoter of autophagy (49.4±2.0%). Immunoblot analyses showed that TNF/zVAD significantly increased the level of LC3-II. The accumulation of LC3-II protein was not further increased by bafilomycin A1 (100 nM), an inhibitor of lysosomal degradation, indicating that accumulation of LC3-II by TNF/zVAD reflected suppression of autophagic flux. Immunoprecipitation experiments revealed that TNF/zVAD reduced interaction between LC3-II and p62, an autophagy adaptor protein, and conversely increased interaction between RIP1 and p62. The changes in p62 interactosomes by TNF/zVAD were reversed by pretreatment with rapamycin. On the other hand, TNF/zVAD-induced cell death when p62 was knocked down was still sensitive to necrostatin-1, indicating that a p62-independent pathway is also involved in RIP1-mediated necroptosis.
Conclusions: Suppression of autophagic flux by p62 sequesteration from p62-LC3-II complexes and its recruitment to RIP1 promotes RIP1-dependent necroptosis by TNF/zVAD.
Author Disclosures: M. Ogasawara: None. T. Yano: None. T. Miki: None. M. Tanno: None. A. Kuno: None. H. Kouzu: None. T. Tobisawa: None. S. Muratsubaki: None. K. Ohno: None. K. Nishizawa: None. S. Ishikawa: None. T. Miura: None.
- © 2015 by American Heart Association, Inc.