Abstract 13895: HOE642 Used During Resuscitation Induced Obviously Adverse Hemodynamic Effects Which May Be Improved by Hypothermia
Introduction: HOE642, the sodium-hydrogen exchanger inhibitor, can delay the opening of mitochondrial permeability transition pore (mPTP) and reduce mitochondrial-derived superoxide production. It was reported HOE642 improved neurological recovery after prolonged ischemia.
Hypothesis: We sought to find the effects of HOE642 with or without hypothermia on resuscitation in rats.
Methods: Thirty male SD rats (240-340g) were included. Cardiac arrest was induced by 8 min asphyxia. Rats were divided into 4 groups: (1) normothermia group (N group, n=9), rectal temperature (Trec) maintained at 37±0.5°C; (2) hypothermia group (H group, n=7), Trec maintained at 37±0.5°C during cardiac arrest and CPR periods, rats were cooled to 33°C in 3 min after ROSC, Trec maintained at 33±0.5°C for 1 h, rewarmed gradually to 37±0.5°C during the following 1 h; (3) HOE642 group (n=7), Trec maintained the same as the N group, HOE642 was given (1mg/kg, iv) when initiating CPR; (4) hypothermia and HOE642 group (H+HOE642 group, n=7), Trec maintained the same as the H group, HOE642 was given the same as the HOE642 group. Epinephrine (0.01mg/kg) was used when initiating CPR. Epinephrine (1μg) was used to maintain MAP ≥ 60mmHg after resuscitation.
Results: There were no significant difference about the baseline characters, CA time, CPR time, and survival among groups. HOE642 did not influence the rate of ROSC. MAP in the H group (113.4±36.7mmHg) was significantly higher than the HOE642 group (67.4±18.5mmHg) and the H+HOE642 group (69.0±17.6mmHg) when ROSC (Fig. 1). MAP in the H group (77.4±21.1mmHg) was significantly higher than the HOE642 group (55.1±18.4mmHg) after 60 min of ROSC. Epinephrine used after ROSC in the HOE642 group (9.4±5.3μg) was significantly more than the N group (0.9±1.1μg), the H group (1.6±1.8μg), and the H+HOE642 group (2.3±1.6μg).
Conclusions: HOE642 used during resuscitation induced hypotension. Hypothermia may prevent the adverse hemodynamic effects caused by HOE642.
Author Disclosures: J. Dong: None. C. Nie: None. M. Chen: None. L. Hou: None. F. Han: None.
- © 2015 by American Heart Association, Inc.