Abstract 13533: Impact of Cola Drinking on the Cardiorenal Axis in Rats
Introduction. The cardiorenal syndrome is a constellation of interactive cardiac and renal risk factors. We reported that chronic cola drinking induces metabolic syndrome and pro-oxidative metabolism in rats.
Hypothesis. To evaluate whether chronic cola drinking affects cardiorenal integrity in relation with oxidative metabolism and local inflammation in rats.
Methods. Male Wistar rats drank ad libitum regular cola (C, n=12) or water (W, n=12). Measures/recordings: body weight (weekly), nutritional data (twice a week); at 0 mo and 6 mo: plasma glucose, cholesterol fractions, TG, uric acid, creatinine (enzymocolorimetry), coenzyme Q-10 (RP-HPLC-ED), SBP (plethysmography), echocardiograms (awake rats). At 6 mo euthanasia was performed. The heart and kidneys were excised and processed for histopathology and immunohistochemical IL-6 and TNFα staining. Glomerulosclerosis and histopathology score were semiquantitatively evaluated. ANOVA, Bonferroni t-test, Pearson r, p<0.05 significant.
Results. Compared with W, C rats showed: I). overweight (8%, p<0.05), hyperglycemia (11%, p<0.05), hypertriglyceridemia (2-fold, p<0.001), higher atherogenic index in plasma (2-fold, p<0.01), lower Q10 levels (-55%, p<0.05); II). higher LV diastolic diameter (+9%, p<0.05) and volumes (diastolic 26%, p<0.01; systolic 24%, p<0.05), posterior wall thinning (-8%, p<0.05) and larger cardiac output (24%, p<0.05); III). glomerulosclerosis (21%, p<0.05), higher histopathological score (13%, p<0.05), IL-6 (7-fold, p<0.001) and TNFα (4-fold, p<0.001) expression; IV). Left ventricle dimensions were strongly correlated with IL-6 (74%, r=0.86, p<0.001) and TNFα (52%, r=0.72, p<0.001). Controlling for either or both TG and Q10reduced the strength of correlations or nearly abolished them.
Conclusions. Cardiac remodeling was associated with increased pro-inflammatory cytokines and renal damage. Cardiorenal association was highly dependent on hypertriglyceridemia and Q10 level. Hypertriglyceridemic lipotoxicity in a context of defective antioxidant and anti-inflammatory protection due to low Q10 level might be involved in the cardiorenal syndrome induced by chronic cola drinking in rats.
Author Disclosures: M. Otero-Losada: None. H. Gómez Llambí: None. A. Müller: None. G. Ottaviano: None. G. Cao: None. G. Ambrosio: None. J. Milei: None.
- © 2015 by American Heart Association, Inc.