Abstract 10402: Early Reduction of Myocardial Reactive Oxygen Species Production by Postconditioning in a Porcine Model of Cardiac Arrest and Resuscitation
Introduction: Ischemic (IPoC) and anesthetic postconditioning (APoC) during cardiopulmonary resuscitation (CPR) are effective interventions to improve hemodynamic outcome and survival in a porcine model of prolonged cardiac arrest. We hypothesized that both strategies decrease the myocardial production of reactive oxygen species (ROS) early during reperfusion.
Methods: After 15 min of untreated ventricular fibrillation (VF), 22 pigs were randomized to receive mechanical CPR for 4 min without (control) or with IPoC (three 20-sec CPR interruptions) or APoC with low- (2 Vol%) or high-dose (4 Vol%) sevoflurane for 3 min before euthanasia and organ harvest. Left ventricular myocardial tissue was immediately frozen in liquid nitrogen and stored at -80°C until electroparamagnetic resonance (EPR) measurements were performed to quantify ROS. Data are mean±SEM. Statistics: ANOVA with SNK-post hoc testing, alpha = 0.05.
Results: EPR spectral changes showed that high-dose APoC (10±1 arbitrary units) and IPoC (15±4) reduced reactive oxygen species normalized to dry tissue weight compared to untreated control animals (33±2); low-dose APoC (34±4) did not.
Conclusions: Our data show that the cardio- and neuroprotective strategies of IPoC and APoC result in decreased myocardial ROS production that can be detected as early as 4 min after reintroduction of blood flow by mechanical CPR; the effective concentration of sevoflurane, however, has to be high enough to achieve APoC. Our findings underline the importance of early organ-protective strategies and contribute to our understanding of the mechanisms of these important clinical interventions to improve outcome and survival after cardiac arrest.
Author Disclosures: A.E. Dikalova: None. S.I. Dikalov: None. M.M. Salzman: None. Q. Cheng: None. T.R. Matsuura: None. J.A. Bartos: None. D. Yannopoulos: None. M.L. Riess: None.
- © 2015 by American Heart Association, Inc.