Abstract 10110: Effects of Inorganic Nitrate on Endothelial Dysfunction Induced by Periodontitis
Introduction: Evidence supports an association between periodontal disease and cardiovascular disease (CVD) risk. Periodontal disease is thought to cause vascular dysfunction contributing to atherogenesis and resulting in an increased risk of stroke and coronary heart disease. Safe therapeutics that might improve vascular function in such patients to reduce risk of CVD is a current unmet need. It is thought that a critical step in the increased CVD risk relates to a periodontitis-induced endothelial dysfunction. Evidence suggests that the intervention of dietary nitrate, to elevate circulating nitrite levels, improves endothelial function. We investigated whether dietary nitrate might provide a method for prevention or reversal of the endothelial dysfunction induced by periodontitis.
Methods: Male C57BL6 mice were either untreated or randomly assigned to receive KNO3 or KCl (15 mmol/L; in the drinking water) either 7 days prior or 7 days post ligature placement (periodontitis) or a sham procedure. After 14 days blood samples were collected for assessment of circulating cell types and activation state using flow cytometry. The vasoreactivity of aortic rings were assessed ex vivo. Maxillae were removed for alveolar bone loss measurement.
Results: When compared to sham animals, ligature-induced periodontitis resulted in bone loss (~2-fold increase in the distance cement enamel junction and the alveolar crest, p<0.001, n=9). This was associated with neutrophilia (n=9, P<0.05) and endothelial dysfunction, reflected by a reduction of acetylcholine-induced relaxation (~20% suppression of the Max response, p<0.05, n=5). Vascular responses to the endothelium-independent vasorelaxant, spermine NONOate, were unchanged. Dietary nitrate treatment improved endothelial function when given either as prophylaxis (p<0.01, n=5) or reversal therapy (p<0.01, n=5). These improvements were not associated with any changes in ligature-induced bone loss but were associated with reduced numbers and activation of circulating neutrophils.
Conclusion: Dietary nitrate improves vascular function in periodontitis in mice and thus may represent an alternative treatment in the cardiovascular complications associated with this condition.
Author Disclosures: D. Fernandes: None. R. Khambata: None. A. Ahluwalia: None.
- © 2015 by American Heart Association, Inc.