Abstract P029: Sucralose Promotes Increase in Fat Accumulation in Human Mesenchymal Stem Cells
Background: Artificial sweeteners are extensively used nowadays as a non-caloric sugar alternative. They are sweeter than sugar, with a presumed high safety profile, hence commonly promoted in weight loss and weight maintenance programs as a sugar substitute. However, recent studies showed that saccharin and acesulfame potassium may actually increase adipogenesis. Here we choose to study the effect of Sucralose on Mesenchymal Stem Cells (MSCs), which was never been tested before. MSCs are multipotent cells which can differentiate to adipocytes, myoblasts, osteoblasts or chondroblasts. We were interested to note if presence of sucralose promotes adipocyte formation. Methods: We cultured MSCs in Normal Glucose DMEM media (5.5mM) or in Adipogenic Media (Lonza Inc., 5.5mM) with or without 0.00mM, 0.45mM or 4.5mM of Sucralose for a total of 6 days. At the end of day 6, cells were stained with Oil Red O stain (lipolysis). Cells were lysed post staining and absorbance of the assimilated dye was measured using a plate reader at 520 nm. Non-stained cells from each media and Sucralose concentration were also lysed as control and RNA was collected for RT-PCR to measure oxidation, inflammation and adipogenesis gene expression estimation. Results: Before lysing the cells for absorbance readings, cells were observed under the microscope for phase contrast image. As we increased the Sucralose concentration, (10-fold) a higher number of fat droplets was observed in MSCs cultured. Moreover, absorbance measurements showed that adipogenesis increased by 1.8 folds as we increased the Sucralose concentration from 0.00mM to 0.45mM. It was also increased by 2.85 folds as we increased the concentrations from 0.00mM to 4.5mM in cells cultured in both Normal Glucose and Adipogenic Media. RT-PCR results for oxidation genes ( Catalase, Superoxide dismutase 1, 2 and 3) , inflammation (TNF, IL-6) and adipogenesis( leptin, adiponectin, PPAR-g, FABP-4, c/EBP alpha and c/EBP beta) are pending. In summary, sucralose appears to promote fat accumulation by Oil Red O stain quantification. RT-PCR studies will confirm if adipogenic genes follow a similar pattern of up-regulation. Conclusion: Our studies indicate that sucralose may promote fat accumulation and warrants further cellular and animal model studies
Author Disclosures: S. Sen: None. C. Rouphael: None. S. Houston: None.
- © 2015 by American Heart Association, Inc.