Abstract 9446: Differential Gene Expression of C-Type Natriuretic Peptide and Its Related Molecules in Ischemic and Dilated Cardiomyopathy: A New Option in the Management of Heart Failure
Introduction: There have been few studies on C-type natriuretic peptide (CNP) in heart failure (HF). Our objective was to evaluate the potential role of the CNP gene (NPPC) and its related molecules in HF using RNA sequencing (RNA-Seq). We also studied other natriuretic peptides (NP)-related molecules in the left ventricular (LV) tissue of HF patients.
Methods: RNA-Seq analysis was performed in 32 human LV tissue samples obtained from 8 control donors (CNT) and patients with dilated (DCM, n=12) and ischemic cardiomyopathy (ICM, n=12) undergoing cardiac transplantation.
Results: Compared with CNT, we found that NPPC mRNA levels (2.28-fold) and its enzyme FURIN (-1.37-fold) were altered only in the ICM group, while its clearance receptor NPR3 was altered in DCM (1.55-fold) and ICM (1.58-fold). In contrast, we noted that NPPB (BNP gene) was upregulated and its enzyme CORIN was downregulated in both groups, while its receptor NPR1 was downregulated only in the DCM group. We validated these results using Real-Time quantitative PCR, increasing the total number of LV samples to 50. Furthermore, NPPC mRNA levels were inversely related to LV function in ICM (r=-0.641, p<0.05).
Conclusion: This study shows a differential change in the CNP gene expression in heart tissue of patients with DCM and ICM. NPPC and its endoprotease FURIN change their expression only in the ischemic LV tissue. NPPC is significantly related to decreased LV function in ICM. These results could provide new etiology-specific diagnostic and therapeutic options in HF.
Author Disclosures: M. Rivera: None. E. Tarazon: None. A. Ortega: None. C. Gil Cayuela: None. J. Triviño: None. E. Rosello: None. M. Garcia Manzanares: None. M. Molina: None. A. Martinez: None. I. Sanchez Lazaro: None. L. Almenar: None. F. Lago: None. L. Martinez Dolz: None. I. Moreno: None. A. Perello: None. V. Bertomeu: None. J. Gonzalez Juanatey: None. J. Montero: None. A. Salvador: None. M. Portoles: None.
- © 2014 by American Heart Association, Inc.