Abstract 326: Total Liquid Ventilation Offers Ultrafast and Whole-Body Cooling in Large Animals
Introduction: Total liquid ventilation with perfluorocarbons (TLV) has been shown to cool down the entire body very rapidly (<10-15 min) in rabbits after cardiac arrest.
Hypothesis: We hypothesized that TLV could also induce rapid and whole-body cooling in larger animals under normal conditions (beating hearts) or during cardiopulmonary resuscitation (CPR).
Methods: In a first set of experiments, anesthetized pigs of 20-30 kg were randomly maintained under conventional gas ventilation or submitted to TLV using a specific prototype for large animals. TLV was instituted using perfluoro-N-octane at 15°C (5 cycles/min; tidal volume = 15 ml/kg; end-expiratory volume = 15 ml/kg). In a second set of experiments, pigs were submitted untreated ventricular fibrillation during 15 min. Cardiopulmonary resuscitation (CPR) was initiated using an automated device (Thumper ®). One group was maintained under conventional CPR with the continuous compression ventilation mode (Control group). Another group was switched to TLV as previously described while pursuing CPR (TLV group).
Results: In beating heart animals (step 1), the target temperature of 33°C was achieved within 15 min in all sites of temperature measurement in the TLV group (e.g. rectal and tympanic temperatures as illustrated in Figure; left panel). In comparison, no passive cooling was observed in the Control group. After cardiac arrest (step 2), a passive cooling was conversely observed after cardiac arrest in the Control group (Figure, right panel). In the TLV group, cooling was accelerated and the target temperature was achieved within 20 min. Hemodynamic parameters were not different in the Control and TLV groups.
Conclusion: TLV induces a very rapid cooling of the entire body in large animals with beating hearts but also during CPR. This approach could be relevant to provide ultra-rapid cooling independently of body weight.
Author Disclosures: R. Tissier: Research Grant; Modest; Grant ANR (France) - ABYSS. F. Lidouren: None. A. Hutin: None. M. Chenoune: None. A. Seeman: None. M. Kohlhauer: None. L. Lotteau: Employment; Significant; Bertin (France). Research Grant; Significant; Grant ANR (France) - ABYSS. J. Ricard: None. P. Carli: None. B. Vivien: None. B. Ghaleh: None. A. Berdeaux: Research Grant; Modest; Grant ANR (France) - ABYSS.
- © 2014 by American Heart Association, Inc.