Abstract 210: Inhalation of H2 Gas Starting After Resuscitation Under Normoxia Improves Neurological Outcomes in a Rat Cardiac Arrest Model
Background: We previously described that inhalation of H2 gas in the presence of hyperoxia, starting at the beginning of cardiopulmonary resuscitation (CPR) and given for 2 h after return of spontaneous circulation (ROSC), significantly improves the functional status of brain and heart in a rat cardiac arrest model. Here, we examined the potential therapeutic benefit of a delayed application of H2 gas either alone or in combination with targeted temperature management (TTM) starting after ROSC under normoxia in post-CA rats.
Methods and Results: After 6 min of ventricular fibrillation induction, all animals were mechanically ventilated (MV) with 100% O2 and resuscitated. At 5 min after achieving ROSC, animals were randomized into four groups: MV with 26% O2 under normothermia (control group), MV with 26% O2 and 1.3% H2 under normothermia (H2 group), MV with 26% O2 under TTM (TTM group), MV with 26% O2 and 1.3% H2 under TTM (TTM + H2 group). The survival rate at 7 d after ROSC was 38.4% (control group), 71.4% (H2 group and TTM group), and 85.7% (TTM+H2 group). The motor activity and spatial working memory at 7 d after ROSC was better in the TTM+H2 group than the TTM group. Consistent with this, degenerative changes in the neuronal processes and activation of microglia in the hippocampus CA1 at 7d after ROSC was less in the TTM+H2 group than the TTM group.
Conclusions: A delayed application of H2 gas therapy starting after ROSC under normoxia improves neurological outcomes independently of TTM.
Author Disclosures: K. Hayashida: None. M. Sano: None. N. Kamimura: None. T. Yokota: None. M. Suzuki: None. S. Ohta: None. K. Fukuda: None. S. Hori: None.
- © 2014 by American Heart Association, Inc.