Abstract 20637: Changes in Gene Expression Profile of Porcine Myocardium After Repetitive Ischemia/Reperfusion
Introduction: Gene expression profile of the myocardium after repetitive ischemia/reperfusion (I/R) was analyzed under experimental conditions.
Hypothesis: This animal model simulates human pre-infarction angina pectoris.
Methods: Domestic pigs (n=20) underwent closed chest repetitive (3x5min cycle) I/R by percutaneous balloon occlusion/deflation of the mid LAD. Sham intervention was performed in controls. After 5h and 24h, myocardial samples were harvested from the entire heart (totally 56 samples equally distributed) from the apex to the heart basis localized by using anatomical landmarks. Next generation sequencing (NGS) of the ischemic, border zone and remote myocardium was performed to identify crucial pathways involved in myocardial protection. The hypoxia inducible factor-1 (HIF-1alpha), Caspase-3, GATA4 and myocyte enhancer factor 2C (MEFC2) gene expression levels were analysed using quantitative real-time PCR (rtPCR) and displayed in 2D polar maps.
Results: NGS resulted in significant differences in gene expressions between regions of the LAD at both time points (5h and 24h) in pathways involving cell survival, cell differentiation, muscle function and energy metabolism after conditioning I/R (Fig. 1). Repetitive I/R resulted in a rapid increase in HIF-1alpha and Caspase-3 expression in the ischemic area and border zone, with moderate increase in MEF2c. The GATA4 expression was more pronounced 24h after I/R (Fig. 2).
Conclusions: Short periods of repetitive I/R without infarction cause alteration in gene expression profile of the myocardium, which might be responsible for the protective effect against subsequent infarction and necrosis.
Author Disclosures: N. Pavo: None. D. Macejovska: None. K. Zlabinger: None. D. Pils: None. G. Maurer: None. M. Emmert: None. S. Hoerstrup: None. M. Gyöngyösi: None.
- © 2014 by American Heart Association, Inc.