Abstract 18267: Effect of the Stress by Supercooling on Cell Proliferation and Immunogenicity of Transplanted Human Induced Pluripotent Stem (iPS) Derived Cardiomyoctes
BACKGROUND: Cell preservation is essential for cell transplantation. I examined the effects of the stress by supercooling on the transplantation of human induced pluripotent stem (iPS) derived cardiomyoctes.
METHODS: Human iPS derived cardiomyoctes were cultured for 20 days (control group), cryopreserved by supercooling macine for 1 week, and rapidly thawed and cultured for 20 days. Proliferation was compared among control and cryopreserved human iPS derived cardiomyoctes by constructing growth curves. Growth factors, cytokines, biochemical features, and cell cycle phase were measured immediately before and after cryopreservation by supercooling, and immunogenicity was evaluated from growth curves generated from human iPS derived cardiomyoctes after 7 days in mixed-lymphocyte culture. Control or cryopreserved human iPS derived cardiomyoctes were transplanted into pig connective tissues and evaluated histologically 2 weeks later.
RESULTS: Cryopreserved human iPS derived cardiomyoctes proliferated more effectively than control cells (P<0.001). Levels of basic fibroblast growth factor and transforming growth factor-β1 were significantly higher (P<0.001), and those of interleukin (IL)-6 and IL-8 were significantly lower after cryopreservation (P<0.001). Fewer peripheral blood lymphocytes were produced in cryopreserved cells than in noncryopreserved cells (P=0.01), and the cell cycle phase of cryopreserved human iPS derived cardiomyoctes shifted primarily to G2 + M from G1 + G0. Noncryopreserved and cryopreserved human iPS derived cardiomyoctes both survived in connective tissue.
CONCLUSION: The stress by supercooling can increase the proliferation of human iPS derived cardiomyoctes and also reduce the immunogenicity of the human heart cells.
Author Disclosures: H. Moriguchi: None.
- © 2014 by American Heart Association, Inc.