Abstract 18066: Elevated Levels of Circulating MicroRNA-223, MicroRNA-127 and MicroRNA-146a are Associated with Risk of Near Future Cardiovascular Events in Asymptomatic Individuals
Background: MicroRNAs (miRNAs) are small regulatory RNAs that are stable in plasma and may have utility as diagnostic markers in cardiovascular disease.
Aim: This study investigated whether circulating miRNAs predict risk of incident cardiovascular events in the general population, by analyzing associations between plasma levels of 10 miRNAs and fatal/non-fatal cardiovascular events in asymptomatic individuals selected from a community based cohort.
Methods: miRNA levels were measured in 71 individuals, 37 who had a cardiovascular event within 1 year (mean age=70 ±10 years, 65% male) and 34 matched controls without events (68 ±9 years, 59% male). miRNAs were selected from those previously associated with incident myocardial infarction (miR-126 -197 -223), unstable plaque (miR-127 -133a -145) or stable coronary artery disease (miR-142 -146a -323a -652). Associations between miRNAs, NT-proBNP, Framingham risk score and cardiovascular events were tested with Cox regression and log-rank tests.
Results: All miRNAs except miR-323a were positively associated with cardiovascular events, independent of NT-proBNP and Framingham risk score, with miR-223 showing the strongest association (hazard ratio [95% confidence interval] upper vs lower tertile: miR-223 3.7 [1.6-8.7] p=0.002; miR-127 3.7 [1.6-8.5] p=0.003; miR-146a 3.5 [1.4-8.5] p=0.006; miR-142 3.6 [1.5-8.6] p=0.005; miR-197 3.4 [1.4-8.1] p=0.007; miR-145 1.9 [1.2-2.9] p=0.007; miR-652 2.9 [1.2-6.9] p=0.017; miR-133a 2.5 [1.1-5.5] p=0.027; miR-126 2.4 [1.0-5.4] p=0.041). All miRNAs were correlated with ≥1 other miRNA (Pearson correlation >0.6, p<0.001) and no miRNA remained predictive of events once miR-223 was included in the model. A risk score combining miR-223, miR-127 and miR-146a (Figure 1) was a stronger predictor than miR-223 alone.
Conclusions: These data suggest that circulating miR-223, miR-127 and miR-146a may improve prediction of cardiovascular events in asymptomatic individuals.
Author Disclosures: A.P. Pilbrow: None. C.M. Frampton: None. A.M. Richards: None. R.W. Troughton: None. V.A. Cameron: None.
- © 2014 by American Heart Association, Inc.