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Abstracts and presentations are embargoed for release at date and time of presentation or time of AHA/ASA news event. Failure to honor embargo policies (http://newsroom.heart.org/newsmedia/embargo-policy) will result in the abstract being withdrawn and barred from presentation.
Core 5. Myocardium: Function and FailureSession Title: Emerging Technologies

Abstract 17092: Intravascular Integrated OCT/NIRF Molecular Imaging with a Novel Mannose Receptor Targeting NIRF Probe to Identify Macrophage-rich High-Risk Plaques

Jae Joong Lee, Kyeongsoon Park, Han Saem Cho, Min Woo Lee, Jun Woo Song, Dong Joo Oh, Wang-Yuhl Oh, Hongki Yoo, Jin Won Kim
Circulation. 2014;130:A17092
Jae Joong Lee
Cardiology, Korea Univ Guro Hosp, Seoul, Korea, Republic of
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Kyeongsoon Park
Div of Bio-imaging, Korea Basic Science Institute, Chuncheon, Korea, Republic of
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Han Saem Cho
Mechanical Engineering, KAIST, Daejeon, Korea, Republic of
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Min Woo Lee
Biomedical Engineering, Hanyang Univ, Seoul, Korea, Republic of
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Jun Woo Song
Cardiology, Korea Univ Guro Hosp, Seoul, Korea, Republic of
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Dong Joo Oh
Cardiology, Korea Univ Guro Hosp, Seoul, Korea, Republic of
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Wang-Yuhl Oh
Mechanical Engineering, KAIST, Daejeon, Korea, Republic of
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Hongki Yoo
Biomedical Engineering, Hanyang Univ, Seoul, Korea, Republic of
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Jin Won Kim
Cardiology, Korea Univ Guro Hosp, Seoul, Korea, Republic of
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Abstract

Background: Macrophage-rich active inflammatory plaques are at high-risk associated with acute coronary events. In current study, we newly developed a near-infrared fluorescence (NIRF) probe targeting mannose receptors which are expressed on plaque macrophages, and combined it with a fully integrated OCT (optical coherence tomography)/NIRF structural-molecular imaging to identify macrophage-rich high-risk plaques in coronary-sized vessels.

Methods and Results: A novel mannose receptor targeting probe was synthesized by chemically conjugating thiolated glycol chitosan with mannose-PEG-maleimide, and then further labeled it with NIRF emitting cyanine7 (ex/em 750 and 773nm). I.V. injection of the NIRF-nanoprobe at a dose of 10 mg/kg into balloon injured rabbits fed with high cholesterol diet was done 48 hours prior to imaging. In vivo OCT/NIRF imaging was performed in the infrarenal aorta and iliac artery at a high pullback speed (20mm/sec) just under contrast flushing, followed by ex vivo fluorescence reflectance imaging (FRI). NIRF macrophage signals in vivo were simultaneously enhanced only in the plaque lesions of the rabbit arteries, which morphology was clearly identified by OCT imaging (figure). Both in vivo and ex vivo imaging demonstrated a significant correlation (TBR) (p<0.05). Fluorescence microscopy and immunohistochemical staining of the corresponding plaque sections showing enhanced NIRF signals revealed a good colocalization with macrophage subsets.

Conclusions: Mannose receptor targeting NIRF probe combined with a dual-modal OCT/NIRF catheter system was able to specifically image macrophage-rich high-risk plaques in vivo in coronary-sized rabbit arteries. This novel imaging approach could have the potential to assess active plaque inflammation and offer a new avenue to identify high-risk coronary plaques prone to rupture.


Embedded Image
  • Coronary artery disease
  • Atherosclerosis
  • Plaque
  • New technology
  • Author Disclosures: J. Lee: None. K. Park: None. H. Cho: None. M. Lee: None. J. Song: None. D. Oh: None. W. Oh: None. H. Yoo: None. J. Kim: None.

  • © 2014 by American Heart Association, Inc.
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    Abstract 17092: Intravascular Integrated OCT/NIRF Molecular Imaging with a Novel Mannose Receptor Targeting NIRF Probe to Identify Macrophage-rich High-Risk Plaques
    Jae Joong Lee, Kyeongsoon Park, Han Saem Cho, Min Woo Lee, Jun Woo Song, Dong Joo Oh, Wang-Yuhl Oh, Hongki Yoo and Jin Won Kim
    Circulation. 2014;130:A17092, originally published November 14, 2014

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    Abstract 17092: Intravascular Integrated OCT/NIRF Molecular Imaging with a Novel Mannose Receptor Targeting NIRF Probe to Identify Macrophage-rich High-Risk Plaques
    Jae Joong Lee, Kyeongsoon Park, Han Saem Cho, Min Woo Lee, Jun Woo Song, Dong Joo Oh, Wang-Yuhl Oh, Hongki Yoo and Jin Won Kim
    Circulation. 2014;130:A17092, originally published November 14, 2014
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