Abstract 16280: Early Regional Neuroinflammatory Profile After Asphyxial Cardiac Arrest Vs. Ventricular Fibrillation Cardiac Arrest Model in Rats
Introduction: The post-resuscitative care for survivors of cardiac arrest (CA) is independent of the etiology of CA. However, significant differences in outcomes from asphyxial CA (ACA) vs. ventricular fibrillation CA (VFCA) exist.
Hypothesis: We hypothesized that early systemic and regional neuroinflammatory cytokine response could differ between ACA vs. VFCA.
Methods: Adult male rats were subjected to 10 min asphyxia or 10 min VFCA (n=8/group). Three hours after return of spontaneous circulation (ROSC), systemic (plasma) and regional brain cytokine profile was assessed in four selectively vulnerable brain regions: cerebellum (CEREB), cortex (CTX), hippocampus (HIP) and striatum (STRI) using 10-plex cytokine array (Luminex).
Results: Asphyxia resulted in ACA with a no-flow time of ~ 6±1 min vs. 10 min in VFCA. Lactate levels were higher after VFCA vs. ACA (Table 1). There were no differences in plasma cytokines, or brain interleukin (IL)-4, IL-6 or GM-CSF. Globally, IFNg was higher in ACA vs. VFCA, while IL-1a and IL-12 were higher in VFCA vs. ACA (all p<0.05), with significant regional differences (Fig 1) that identified striatum as a selective neuroinflammatory target in both ACA and VFCA models, and cerebellum in ACA.
Conclusions: Despite shorter no-flow time, ACA resulted in similar systemic and brain cytokine profile as VFCA. However, selected brain cytokines showed regional and insult-specific differences that could contribute to differences in outcome. Assessment by immunohistochemistry to identify the cell types involved for these inflammatory markers could be important to defining new therapeutic targets.
Author Disclosures: T. Uray: Research Grant; Significant; Laerdal Foundation for Acute Medicine, Max Kade Foundation, Inc.. C. Dezfulian: None. A. Palmer: None. J.P. Stezoski: None. K. Janesko-Feldman: None. P.M. Kochanek: None. T. Drabek: None.
- © 2014 by American Heart Association, Inc.