Abstract 16120: Extracellar Matrix Preserving Decellulerized Heart Valve Maintain Valvular Function in vivo by Enhancing Functional Cell Recellularization
Background: Maintanance of the valve function and the growth of the implanted heart valves are of great concerns for the treatment of heart valve insufficiency in congenital heart disease. Recently, several reports suggest that the fresh, not for freezing, decellularized heart valves maintained their structure and function after the implantation. However, the detailed process of recellurlarization of scaffold tissue after implantation remain unclear. We examined the long-term function of fresh decellularized heart valves implanted in the pulmonary valve position of porcine hearts in vivo, together with the recellularization and growth potential of the valve scaffolds.
Methods: We used the pulmonary heart valve from heart transplant recipient heart or from mini-pigs. The valve were decellularized with 0.5% sodium deoxycholate and 0.5% sodium dodecyl sulfate with shaking condition for 36 hours. We implanted theses valves to mini-pigs in orthotropic position with cardiopulmonary machine(human heart valve: 5(xenograft), pig heart valve 5 (allograft)),without immunosuppressant drugs. Valve function was examined by UCG and MRI every month after implantation and by histological examination at 8 months after implantation..
Results: The nuclei of the cells were removed completely and extracellular matrix components such as collagen I and III were maintained in the decellularized pulmonary valves. UCG and MRI examination revealed that valve function was maintained in all cases. We did not detect calcification in implanted valves at 8 months after the implantation. The leaflet was covered with CD31-positive cells and synthetic-type smooth muscle cells, which expressed both vimentin and SMA at 8 months after implantation. Also, vimentin positive-cells surrounded collagen I were also detected at the graft border.
Conclusions: The fresh decellularized heart valve may be recelluralized by recipient originated somatic stem cells which are responsible for production of ECM proteins and endothelialization on leaflet or artery wall after in vivo implantation, promising a long term maintenance of valvular function.
- Tissue engineering
- Congenital heart disease
- Regenerative medicine stem cells
- Pulmonary valve
- Stem cell therapy
Author Disclosures: H. Ozawa: None. S. Miyagawa: None. S. Fukushima: None. A. Harada: None. E. Ito: None. A. Saitou: None. T. Ueno: None. K. Toda: None. T. Kuratani: None. Y. Sawa: None.
- © 2014 by American Heart Association, Inc.