Abstract 14988: Involvement of Adventitial Vasa Vasorum and Sympathetic Nerves in Coronary Hyperconstricting Responses After Drug-eluting Stent Implantation in Pigs in vivo
Purpose: We have previously demonstrated that adventitial inflammation plays an important role in the pathogenesis of coronary vasospasm in porcine models. The adventitia harbors a variety of components with potent modulation of vascular tone, including vasa vasorum (VV) and sympathetic nerve fibers (SNFs). We tested our hypothesis that adventitial VV and SNFs are involved in the pathogenesis of coronary hyperconstricting responses after DES implantation in pigs in vivo.
Methods: We randomly implanted 3 different types of DES, sirolimus-eluting stents (SES, 1st generation DES), everolimus-eluting stents (EES, 2nd generation DES) and biolimus A9-eluting stents (BES, new generation DES), into the left porcine coronary arteries (n=12 each). After 1 month, coronary responses to intracoronary serotonin (10 and 100 μg/kg) were examined by coronary angiography. After euthanasia, stented vessels and untreated vessels (as controls) were harvested for immunohistochemistry of adventitial VV (von Willebrand factor, vWF) and SNFs (tyrosine hydroxylase, TH).
Results: Coronary vasoconstricting responses to serotonin were significantly enhanced at the edges of the SES and the EES sites, associated with increased adventitial densities of VV (vWF) and SNFs (TH) as compared with the control sites and the BES edges (all P<0.05) (Figure). There were highly significant correlations between SNFs and VV (R=0.64, P<0.0001) and between SNFs and vasoconstricting responses (R=0.47, P<0.005).
Conclusions: These results indicate that adventitial VV and SNFs may be involved in the pathogenesis of DES-induced coronary hyperconstricting responses, which are less pronounced with new generation BES.
Author Disclosures: H. Uzuka: None. Y. Matsumoto: None. K. Nishimiya: None. M. Hirano: None. T. Ogata: None. T. Shindo: None. K. Hanawa: None. Y. Hasebe: None. K. Hao: None. R. Tsuburaya: None. K. Ito: None. J. Takahashi: None. H. Shimokawa: None.
- © 2014 by American Heart Association, Inc.