Abstract 14804: Transplantation of Human Umbilical Cord Blood Mononuclear Cells Improves LV function and Remodeling After a reperfused Myocardial Infarction
Introduction: Human umbilical cord blood cells (hUCBC) contain numerous stem/progenitor cells, which may help repair damaged hearts after acute myocardial infarction (MI). Previous studies of hUCBC injection were performed in models of permanent occlusion of coronary artery mostly using immunocompromised animals. These experimental conditions have less relevance for cell therapy in humans.
Hypothesis: We hypothesized that hUCBC injection would improve left ventricular (LV) structure and function in rats after a reperfused MI.
Methods: The mononuclear fraction was isolated from human cord blood collected after a term delivery. Age-matched normal (without immunosuppression) male Fischer 344 rats underwent a 30-min occlusion of LAD coronary artery followed by reperfusion, and 48 h later received intramyocardial injection of vehicle (PBS) or 1.2x106 hUCBC mononuclear cells. Echocardiography was performed 4 d prior to coronary occlusion/reperfusion (BSL) and at 48 h and 35 d after cell injection. Detailed assessment of cardiac structure and function was performed. Rats were sacrificed at 35 d.
Results: At 48 h, LVEF in both groups was significantly reduced compared with baseline (Figure). At 35 d after cell injection, rats injected with hUCBCs exhibited significantly higher EF compared with PBS-injected rats (Figure). hUCBC-injected hearts also showed significantly smaller increase in LV end-diastolic and end-systolic diameters after 35 d, indicating improved remodeling compared with PBS-injected hearts.
Conclusions: Transplantation of hUCBCs after a reperfused MI in non-immunosuppressed rats attenuates LV dysfunction and improves LV remodeling. These findings support potential therapeutic use of hUCBC transplantation to improve myocardial function and remodeling in humans after a reperfused MI, a common clinical scenario.
Author Disclosures: K. Choksi: None. G. Cheng: None. A. Davani: None. A. Samanta: None. M. Girgis: None. A. Cantilena: None. R. Vincent: None. Y. Yang: None. H.K. Elias: None. C. Weiner: None. B. Dawn: None.
- © 2014 by American Heart Association, Inc.